Ssu72 phosphatase deficiency leads to spindle crossing during the second meiotic division process.

Q3 Medicine
遗传 Pub Date : 2024-06-20 DOI:10.16288/j.yczz.24-047
Jing-Liang Yan, Ling-Ling Ma, Yoshinori Watanabe
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引用次数: 0

Abstract

Ssu72 is a component of the yeast cleavage/polyadenylation factor (CPF) complex, which catalyzes the dephosphorylation of the C-terminal domain (CTD) of RNA polymerase II at S5-P and S7-P. It has been shown that Ssu72 phosphatase is involved in regulating chromosome cohesion during mitosis. To further clarify whether Ssu72 phosphatase affects chromosome separation during meiotic division in Schizosaccharomyces pombe, we utilized green fluorescent protein (GFP) to label centromeres and red fluorescent protein to label microtubule protein Atb2. The entire meiotic chromosome separation process of ssu72∆ cells was observed in real-time under fluorescence microscope. It was found that two spindles of ssu72∆ cells crossed during the metaphase and anaphase of the second meiotic division, and this spindle crossing led to a new type of spore defect distribution pattern. The results of this study can provide important reference significance for studying the roles of phosphatase Ssu72 in higher organisms.

Ssu72 磷酸酶缺乏会导致减数第二次分裂过程中的纺锤体交叉。
Ssu72 是酵母裂解/多腺苷酸化因子(CPF)复合物的一个组成部分,它能催化 RNA 聚合酶 II 的 C 端结构域(CTD)在 S5-P 和 S7-P 处的去磷酸化。研究表明,Ssu72 磷酸酶参与了有丝分裂过程中染色体内聚力的调节。为了进一步弄清Ssu72磷酸酶是否会影响小鼠减数分裂过程中染色体的分离,我们利用绿色荧光蛋白(GFP)标记中心粒,红色荧光蛋白标记微管蛋白Atb2。在荧光显微镜下实时观察了 ssu72∆ 细胞减数分裂染色体分离的全过程。结果发现,su72∆细胞的两个纺锤体在减数第二次分裂的有丝分裂期和无丝分裂期发生了交叉,这种纺锤体交叉导致了一种新型的孢子缺陷分布模式。该研究结果可为研究磷酸酶Ssu72在高等生物中的作用提供重要的参考意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
遗传
遗传 Medicine-Medicine (all)
CiteScore
2.50
自引率
0.00%
发文量
6699
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