How long does the biological effect of a red light-emitting diode last on adipose-derived mesenchymal stem cells?

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Photochemistry and Photobiology Pub Date : 2025-01-01 Epub Date: 2024-06-18 DOI:10.1111/php.13983

Vitor Pocani da Rocha, Barbara Sampaio Dias Martins Mansano, Carolina Fernanda Chaves Dos Santos, Ighor Luiz Azevedo Teixeira, Helenita Antonia de Oliveira, Stella Sousa Vieira, Ednei Luiz Antonio, Maria Cristina de Oliveira Izar, Francisco Antonio Helfenstein Fonseca, Andrey Jorge Serra

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Abstract

This research investigated the duration of the influence of red light-emitting diodes (LED, 630 nm; output power: 2452.5 mW; laser beam: 163.5 cm²; irradiance: 15 mW/cm²; radiant exposure: 4 J/cm²) on different periods after irradiation (6, 12, 24, 48, and 72 h) on adipose-derived mesenchymal stem cells' (AdMSCs) metabolism and paracrine factors. AdMSCs were irradiated three times every 48 h. Twenty-four hours after the last irradiation, there was a higher MTT absorbance, followed by a decrease after 48 h. The cells' secretome showed increased levels of IL-6 and VEGF after 12 and 24 h, but this was reversed after 48 h. Additionally, LED irradiation resulted in higher levels of nitrite and did not affect oxidative stress markers. LED irradiation had significant effects on AdMSCs after 24 h compared to other groups and its control group.

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红色发光二极管对脂肪间充质干细胞的生物效应能持续多久？

本研究探讨了红色发光二极管（LED，630 nm；输出功率：2452.5 mW；激光束：163.5 cm2；辐照度：15 mW/cm2；辐射量：4 J/cm2）在照射后不同时期（6、12、24、48和72 h）对脂肪间充质干细胞（AdMSCs）新陈代谢和旁分泌因子的影响持续时间。最后一次照射24小时后，MTT吸光度升高，48小时后下降。细胞分泌组显示，12小时和24小时后IL-6和血管内皮生长因子水平升高，但48小时后逆转。与其他组及其对照组相比，LED照射在24小时后对AdMSCs有明显影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photochemistry and Photobiology 生物-生化与分子生物学

CiteScore

6.70

自引率

12.10%

发文量

171

审稿时长

2.7 months

期刊介绍： Photochemistry and Photobiology publishes original research articles and reviews on current topics in photoscience. Topics span from the primary interaction of light with molecules, cells, and tissue to the subsequent biological responses, representing disciplinary and interdisciplinary research in the fields of chemistry, physics, biology, and medicine. Photochemistry and Photobiology is the official journal of the American Society for Photobiology.