Biological and prognostic significance of NDRG2 downregulation in oral squamous cell carcinoma.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2024-10-01 Epub Date: 2024-06-17 DOI:10.1111/odi.15045

Chi-Hyun Ahn, Ji-Hoon Kim, Hye-Won Shim, Wui-Jung Shin, Young-Ah Cho, Hye-Jung Yoon

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引用次数: 0

Abstract

Objective: Downregulation of N-myc downstream-regulated gene 2 (NDRG2), a tumor suppressor gene, has been associated with poor clinical outcomes in various cancers. However, the prognostic significance of NDRG2 in oral squamous cell carcinoma (OSCC) remains unknown. This study aimed to evaluate the prognostic value of NDRG2 downregulation in OSCC and to elucidate the mechanism by which NDRG2 is downregulated and the biological role of NDRG2 in tumor progression.

Methods: Immunohistochemical and in silico analyses of NDRG2 expression were performed, and the correlation between NDRG2 expression and clinicopathological data was analyzed. The effect of NDRG2 knockdown on the biological behavior of OSCC cells was investigated and the effect of 5-aza-2'-deoxycytidine (5-aza-dC) on NDRG2 expression was determined.

Results: NDRG2 expression was significantly downregulated and DNA hypermethylation of NDRG2 was frequently found in head and neck SCC, including OSCC. Low NDRG2 expression was significantly correlated with adverse clinicopathological features and worse survival in OSCC. NDRG2 knockdown could enhance the oncogenic properties of OSCC cells. NDRG2 mRNA levels in OSCC cells could be restored by 5-aza-dC.

Conclusion: Downregulation of NDRG2 promotes tumor progression and predicts poor prognosis in OSCC. Therefore, restoration of NDRG2 expression may be a potential therapeutic strategy in OSCC.

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口腔鳞状细胞癌中 NDRG2 下调的生物学意义和预后。

目的：N-myc下游调控基因2（NDRG2）是一种肿瘤抑制基因，它的下调与多种癌症的不良临床预后有关。然而，NDRG2在口腔鳞状细胞癌（OSCC）中的预后意义仍不清楚。本研究旨在评估NDRG2下调在OSCC中的预后价值，并阐明NDRG2下调的机制以及NDRG2在肿瘤进展中的生物学作用：方法：对NDRG2的表达进行免疫组化和硅学分析，并分析NDRG2表达与临床病理数据的相关性。研究了敲除NDRG2对OSCC细胞生物学行为的影响，并确定了5-aza-2'-脱氧胞苷（5-aza-dC）对NDRG2表达的影响：结果：NDRG2表达明显下调，NDRG2的DNA高甲基化在头颈部SCC（包括OSCC）中经常出现。NDRG2的低表达与OSCC的不良临床病理特征和较差的生存率明显相关。敲除NDRG2可增强OSCC细胞的致癌特性。5-aza-dC可恢复OSCC细胞中的NDRG2 mRNA水平：结论：NDRG2的下调会促进肿瘤进展，并预示着OSCC的不良预后。因此，恢复NDRG2的表达可能是OSCC的一种潜在治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.