Optimized sperm selection: a highly efficient device for the isolation of progressive motile sperm with low DNA fragmentation index.

IF 3.2 3区医学 Q2 GENETICS & HEREDITY

Journal of Assisted Reproduction and Genetics Pub Date : 2024-08-01 Epub Date: 2024-06-18 DOI:10.1007/s10815-024-03168-9

Ileana Mateizel, Annalisa Racca, Eleni Aligianni, Elisa Distasi, Yoni Baert, Ingrid Segers, Danijel Jankovic, Celine Schoemans, Koen Wouters, Herman Tournaye, Neelke De Munck

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Abstract

Purpose: To identify the sperm preparation procedure that selects the best sperm population for medically assisted reproduction.

Methods: Prospective observational study comparing the effect of four different sperm selection procedures on various semen parameters. Unused raw semen after routine diagnostic analysis was split in four fractions and processed by four different methods: (1) density gradient centrifugation (DGC), (2) sperm wash (SW), (3) DGC followed by magnetic activated cell sorting (MACS), and (4) using a sperm separation device (SSD). Each fraction was analyzed for progressive motility, morphology, acrosome index (AI), and DNA fragmentation index (DFI).

Results: With DGC as standard of care in intraclass correlation coefficient analysis, only SSD was in strong disagreement regarding progressive motility and DFI [0.26, 95%CI (- 0.2, 0.58), and 0.17, 95%CI (- 0.19, 0.45), respectively]. When controlling for abstinence duration, DFI was significantly lower after both MACS and SSD compared to DGC [- 0.27%, 95%CI (- 0.47, - 0.06), p = 0.01, and - 0.6%, 95%CI (- 0.80, - 0.41), p < 0.001, respectively]. Further comparisons between SSD and MACS indicate significantly less apoptotic cells [Median (IQR) 4 (5), 95%CI (4.1, - 6.8) vs Median (IQR) 5 (8), 95%CI (4.9, - 9.2), p < 0.001, respectively] and dead cells [Median (IQR) 9.5 (23.3), 95%CI (13.2, - 22.4) vs Median (IQR) 22 (28), 95%CI (23.1, - 36.8), p < 0.001, respectively] in the SSD group.

Conclusion: The selection of a population of highly motile spermatozoa with less damaged DNA from unprocessed semen is ideally performed with SSD. Question remains whether this method improves the embryological outcomes in the IVF laboratory.

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优化精子选择：一种用于分离具有低 DNA 破碎指数的进步运动精子的高效装置。

目的：确定精子制备程序，为医学辅助生殖选择最佳精子群：前瞻性观察研究：比较四种不同精子选择程序对精液各种参数的影响。将常规诊断分析后未使用的原始精液分成四个部分，并采用四种不同的方法进行处理：（1）密度梯度离心法（DGC）；（2）精子洗涤法（SW）；（3）密度梯度离心法后磁激活细胞分拣法（MACS）；（4）使用精子分离装置（SSD）。对每部分精子的运动能力、形态、顶体指数（AI）和 DNA 破碎指数（DFI）进行分析：结果：在类内相关系数分析中，以 DGC 为标准，只有 SSD 在进行性活力和 DFI 方面存在较大差异[分别为 0.26，95%CI (- 0.2, 0.58) 和 0.17，95%CI (- 0.19, 0.45)]。在控制戒断持续时间后，与 DGC 相比，MACS 和 SSD 后的 DFI 均显著降低[- 0.27%，95%CI (- 0.47, - 0.06)，p = 0.01，和- 0.6%，95%CI (- 0.80, - 0.41)，p 结论：MACS 和 SSD 在选择高动机人群时，应考虑到戒断持续时间：从未经处理的精液中挑选DNA受损较少、运动能力强的精子是使用SSD的理想选择。但这种方法是否能改善试管婴儿实验室的胚胎学结果，仍是个问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Assisted Reproduction and Genetics 医学-妇产科学

CiteScore

5.70

自引率

9.70%

发文量

286

审稿时长

1 months

期刊介绍： The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species. The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.