Protective effect of rubber seed oil on human endothelial cells

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2024-06-19 DOI:10.1007/s10735-024-10198-1

Yujie Zhang, Fuchuan Huang, Yiran Wu, Linmei Jiao, Yun Wang, Tao Ding

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引用次数: 0

Abstract

Objective

This study was conducted to characterize the antioxidant and anti-inflammatory properties of Rubber Seed Oil (RSO) against atherosclerosis (AS) through the study of the protective effects and mechanisms on human umbilical vein endothelial cells (HUVECs) injury induced by oxidized low-density lipoprotein (ox-LDL).

Methods

HUVECs were treated with RSO, ox-LDL, RSO + ox-LDL, respectively, followed by cell activity testing, levels of IL-1β, IL-6, IL-10, TNF-α, ROS, NO, the mRNA expression of eNOS and protein expression of MCP-1, VCAM-1, eNOS, TLR4, NF-κB p65、p-NF-κB p65.

Results

Compared with the ox-LDL group, cell viability, NO level and the expression of eNOS mRNA significantly increased. and the levels of pro-inflammatory factors such as IL-1β, IL-6, TNF-α, IL-10, ROS were significantly decreased, which was accompanied by decreases in TLR4 mRNA, TLR4, MCP-1, VCAM-1 protein expression, as well as the ratio of NF-κB p-p65/p65 in the group treated with 250 μg/ml ox-LDL + 50 μg/ml RSO, 250 μg/ml ox-LDL + 100 μg/ml RSO, 250 μg/ml ox-LDL + 150 μg/ml RSO.

Conclusions

RSO can reduce the expression of pro-inflammatory mediators, oxidative factors involved in injured vascular endothelial cells, exhibiting anti-inflammatory and antioxidant properties HUVECs exposed to ox-LDL. In addition, it may alleviate endothelial cell damage by inhibiting the TLR4/NF-κB signaling pathway.

Abstract Image

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橡胶籽油对人体内皮细胞的保护作用

研究目的本研究通过研究橡胶籽油（RSO）对氧化低密度脂蛋白（ox-LDL）诱导的人脐静脉内皮细胞（HUVECs）损伤的保护作用和机制，探讨橡胶籽油对动脉粥样硬化（AS）的抗氧化和抗炎特性。方法：分别用 RSO、ox-LDL、RSO + ox-LDL 处理 HUVECs，检测细胞活性、IL-1β、IL-6、IL-10、TNF-α、ROS、NO 水平、eNOS mRNA 表达及 MCP-1、VCAM-1、eNOS、TLR4、NF-κB p65、p-NF-κB p65 蛋白表达：结果：与 ox-LDL 组相比，细胞活力、NO 水平和 eNOS mRNA 表达均显著增加。TLR4 mRNA、TLR4、MCP-1、VCAM-1 蛋白表达减少、此外，250 μg/ml ox-LDL + 50 μg/ml RSO组、250 μg/ml ox-LDL + 100 μg/ml RSO组、250 μg/ml ox-LDL + 150 μg/ml RSO组的NF-κB p-p65/p65比值也有所下降。结论RSO 可降低促炎介质的表达，减少参与损伤血管内皮细胞的氧化因子，对暴露于 ox-LDL 的 HUVECs 具有抗炎和抗氧化作用。此外，它还能通过抑制 TLR4/NF-κB 信号通路减轻内皮细胞损伤。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.