Dynamic Regulation of Cell Mechanotransduction through Sequentially Controlled Mobile Surfaces

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2024-06-18 DOI:10.1021/acs.nanolett.4c01371

Wenyan Xie, Linjie Ma, Peng Wang, Xiaojing Liu, Di Wu, Yuan Lin, Zhiqin Chu, Yong Hou* and Qiang Wei*,

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引用次数: 0

Abstract

The physical properties of nanoscale cell-extracellular matrix (ECM) ligands profoundly impact biological processes, such as adhesion, motility, and differentiation. While the mechanoresponse of cells to static ligands is well-studied, the effect of dynamic ligand presentation with “adaptive” properties on cell mechanotransduction remains less understood. Utilizing a controllable diffusible ligand interface, we demonstrated that cells on surfaces with rapid ligand mobility could recruit ligands through activating integrin α5β1, leading to faster focal adhesion growth and spreading at the early adhesion stage. By leveraging UV-light-sensitive anchor molecules to trigger a “dynamic to static” transformation of ligands, we sequentially activated α5β1 and αvβ3 integrins, significantly promoting osteogenic differentiation of mesenchymal stem cells. This study illustrates how manipulating molecular dynamics can directly influence stem cell fate, suggesting the potential of “sequentially” controlled mobile surfaces as adaptable platforms for engineering smart biomaterial coatings.

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通过顺序控制的移动表面动态调节细胞的机械传导。

纳米级细胞-细胞外基质（ECM）配体的物理特性对粘附、运动和分化等生物过程有着深远的影响。虽然细胞对静态配体的机械响应研究得很透彻，但具有 "自适应 "特性的动态配体呈现对细胞机械传导的影响仍不甚了解。利用可控扩散配体界面，我们证明了配体快速移动表面上的细胞可以通过激活整合素α5β1来招募配体，从而在早期粘附阶段加快病灶粘附的生长和扩散。通过利用对紫外线敏感的锚分子触发配体的 "动静 "转换，我们依次激活了α5β1和αvβ3整合素，显著促进了间充质干细胞的成骨分化。这项研究说明了操纵分子动力学如何直接影响干细胞的命运，表明了 "顺序 "控制移动表面作为工程智能生物材料涂层的适应性平台的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.