Dark septate endophytes enhance the drought tolerance of Haloxylon ammodendron in sterilized and nonsterilized soil

IF 2 3区农林科学 Q3 ECOLOGY

Pedobiologia Pub Date : 2024-07-01 DOI:10.1016/j.pedobi.2024.150971

Qiannan Ye , Xia Li , Junmeng Long , Xueli He

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引用次数: 0

Abstract

Dark septate endophytes (DSEs) are a type of endophytic fungus that commonly colonize plant root systems in extreme environments, and play a role in enhancing resistance to drought stress. To investigate the potential applications of DSEs in improving drought tolerance of desert plants, three DSE strains isolated from Haloxylon ammodendron for strong drought tolerance - Alternaria tellustris (AT), Cladosporium sp. (CL), and Paraphoma radicina (PR) - were screened through pure culture in vitro. Pot experiments of H. ammodendron were then conducted with different DSE, water, and soil treatments. In both sterilized and nonsterilized soil, DSEs showed growth-promoting and drought-resistant properties, with a stronger effect observed under sterilized soil treatment. The results showed that in sterilized soil, AT and CL increased root biomass, total biomass and root shoot ratio under high drought treatment, while PR effectively enhanced branch number and root biomass under normal water treatment. Physiologically, DSEs improved plant drought tolerance by increasing soluble sugar content and superoxide dismutase activity. Notably, DSE inoculation facilitated the uptake and utilization of soil nutrients such as available phosphorus, nitrate nitrogen, and free amino acids by plants in both sterilized and nonsterilized soil. Overall, our study highlights the potential of DSEs in improving drought resistance and promoting the growth of desert plants.

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暗隔内生菌增强了灭菌和非灭菌土壤中哈洛克龙（Haloxylon ammodendron）的抗旱能力

暗隔内生真菌（DSE）是内生真菌的一种，通常定殖于极端环境中的植物根系，在提高抗旱性方面发挥作用。为了研究 DSEs 在提高沙漠植物抗旱性方面的潜在应用，研究人员通过体外纯培养筛选了从 Haloxylon ammodendron 中分离出的三种具有较强抗旱性的 DSE 菌株--Alternaria tellustris（AT）、Cladosporium sp.然后用不同的 DSE、水和土壤处理方法对 H. ammodendron 进行了盆栽实验。在灭菌和非灭菌土壤中，DSEs 都表现出促进生长和抗旱的特性，在灭菌土壤处理中观察到的效果更强。结果表明，在灭菌土壤中，AT 和 CL 在高旱处理下可提高根生物量、总生物量和根芽比，而 PR 在正常水处理下可有效提高分枝数和根生物量。在生理学上，DSE 通过增加可溶性糖含量和超氧化物歧化酶活性来提高植物的抗旱能力。值得注意的是，在灭菌和非灭菌土壤中，接种 DSE 都能促进植物对土壤养分（如可用磷、硝态氮和游离氨基酸）的吸收和利用。总之，我们的研究强调了 DSE 在提高沙漠植物抗旱性和促进其生长方面的潜力。

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来源期刊

Pedobiologia 环境科学-生态学

CiteScore

4.20

自引率

8.70%

发文量

审稿时长

64 days

期刊介绍： Pedobiologia publishes peer reviewed articles describing original work in the field of soil ecology, which includes the study of soil organisms and their interactions with factors in their biotic and abiotic environments. Analysis of biological structures, interactions, functions, and processes in soil is fundamental for understanding the dynamical nature of terrestrial ecosystems, a prerequisite for appropriate soil management. The scope of this journal consists of fundamental and applied aspects of soil ecology; key focal points include interactions among organisms in soil, organismal controls on soil processes, causes and consequences of soil biodiversity, and aboveground-belowground interactions. We publish: original research that tests clearly defined hypotheses addressing topics of current interest in soil ecology (including studies demonstrating nonsignificant effects); descriptions of novel methodological approaches, or evaluations of current approaches, that address a clear need in soil ecology research; innovative syntheses of the soil ecology literature, including metaanalyses, topical in depth reviews and short opinion/perspective pieces, and descriptions of original conceptual frameworks; and short notes reporting novel observations of ecological significance.