The activity of cholinergic neurons in the basal forebrain interferes with anesthesia-arousal process of propofol

IF 2.5 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Neuropeptides Pub Date : 2024-06-14 DOI:10.1016/j.npep.2024.102449

Cai-hua Feng , Xiao-Nan Du , Zhi Wang , Ting Wu , Li-Na Zhang

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引用次数: 0

Abstract

Previous research has demonstrated that basal forebrain (BF) regulates arousal during propofol anesthesia. However, as the BF comprises cholinergic neurons alongside two other types of neurons, the specific role of cholinergic neurons has not been definitively elucidated. In our study, calcium signal imaging was utilized to monitor the real-time activities of cholinergic neurons in the BF during propofol anesthesia. Additionally, we selectively stimulated these neurons to investigate EEG and behavioral responses during propofol anesthesia. Furthermore, we specifically lesioned cholinergic neurons in the BF to investigate the sensitivity to propofol and the induction time. The results revealed that propofol suppressed calcium signals of cholinergic neurons within the BF following intraperitoneal injection. Notably, upon recovery of the righting reflex, the calcium signals partially recovered. Spectral analysis of the EEG elucidated that optical stimulation of cholinergic neurons led to a decrease in δ power underlie propofol anesthesia. Conversely, depletion of cholinergic neurons in the BF enhanced sensitivity to propofol and shortened the induction time. These findings clarify the role of cholinergic neurons in the anesthesia-arousal process, as well as the depth and the sensitivity of propofol anesthesia.

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前脑基底胆碱能神经元的活动干扰异丙酚的麻醉-唤醒过程

以往的研究表明，在异丙酚麻醉过程中，基底前脑（BF）可调节唤醒。然而，由于前脑基底层包括胆碱能神经元和其他两种类型的神经元，胆碱能神经元的具体作用尚未明确阐明。在我们的研究中，我们利用钙信号成像来监测异丙酚麻醉期间 BF 中胆碱能神经元的实时活动。此外，我们还选择性地刺激了这些神经元，以研究异丙酚麻醉期间的脑电图和行为反应。此外，我们还对 BF 中的胆碱能神经元进行了特异性病变，以研究其对异丙酚的敏感性和诱导时间。结果发现，腹腔注射异丙酚后，BF内胆碱能神经元的钙信号受到抑制。值得注意的是，当右反射恢复后，钙信号部分恢复。脑电图的频谱分析阐明，对胆碱能神经元的光刺激导致δ功率下降是异丙酚麻醉的基础。相反，BF 中胆碱能神经元的耗竭增强了对异丙酚的敏感性并缩短了诱导时间。这些发现阐明了胆碱能神经元在麻醉-唤醒过程中的作用，以及异丙酚麻醉的深度和敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuropeptides 医学-内分泌学与代谢

CiteScore

5.40

自引率

6.90%

发文量

审稿时长

>12 weeks

期刊介绍： The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.