Xuesheng Han MS , David Vollmer MS , Xuefei Yan MS , Yahong Zhang MS , Mingfa Zang MPVM , Chenfei Zhang MS , Catherine M. Sherwin MS , Elena Y. Enioutina MS
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引用次数: 0
Abstract
Objectives
Natural killer (NK) cells are important immune system effector cells providing innate defenses against intracellular infections, including viral infections, immune surveillance, and cancer immunoediting. The primary purpose of this study was to investigate whether modified ultra-filtrated colostrum (UC) and hydrolyzed whey (W) products or their combinations with other natural products with reported immunomodulatory properties will stimulate NK cell cytotoxic activity by activation of granzyme B and IFN-γ production.
Methods
The ability of study products to stimulate the cytotoxic activity of human-purified CD56+ NK cells and the production of granzyme B and IFN-γ by activated NK cells was evaluated in the cytotoxic assay.
Results
All study products significantly increased NK-cell cytotoxic activity at an E: T ratio of 20:1. Treatment of cells with UC had a maximal cytotoxic effect at the minimal dose of 10 µg/ml, which exceeded the cytotoxic activity of IL-2. In contrast, the addition of egg yolk (CE) or CE + botanical blend (CEB) to UC resulted in a dose-dependent cytotoxic response with a maximal response at 1000 µg/ml. The maximal activity of blend products was comparable to UC activity. W exerted minimal stimulatory activity on NK cells. The magnitude of granzyme B and IFN-γ production was closely associated with the cytotoxic activity of NK cells stimulated with the study products.
Conclusions
All study products demonstrated stimulatory activity on NK cells, with UC having a maximal effect on NK cell cytotoxicity. The study products can be used as dietary supplements to support NK cell activity in healthy individuals.
目的自然杀伤(NK)细胞是重要的免疫系统效应细胞,可提供针对细胞内感染(包括病毒感染)的先天防御、免疫监视和癌症免疫编辑。本研究的主要目的是调查改良超滤牛初乳(UC)和水解乳清(W)产品或它们与其他据报道具有免疫调节特性的天然产品的组合是否会通过激活颗粒酶 B 和 IFN-γ 的产生来刺激 NK 细胞的细胞毒活性。方法在细胞毒性试验中评估研究产品刺激人类纯化的 CD56+ NK 细胞的细胞毒性活性以及活化的 NK 细胞产生颗粒酶 B 和 IFN-γ 的能力。用 UC 处理细胞时,最小剂量为 10 µg/ml,细胞毒性作用最大,超过了 IL-2 的细胞毒性活性。相比之下,在 UC 中加入蛋黄(CE)或 CE + 植物混合物(CEB)会产生剂量依赖性细胞毒性反应,最大反应剂量为 1000 µg/ml。混合产品的最大活性与 UC 的活性相当。W 对 NK 细胞的刺激活性最小。颗粒酶 B 和 IFN-γ 的产生量与用研究产品刺激的 NK 细胞的细胞毒性活性密切相关。研究产品可用作膳食补充剂,以支持健康人的 NK 细胞活性。
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