Kerala Ayurveda Ostoact Tablet treats osteoporosis in ovariectomized rat model via regulating RANKL/OPG pathway

IF 3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2025-09-01 DOI:10.1016/j.jtcme.2024.06.007

Deepa Mandlik, Rutuja Patil, S. Arulmozhi, Satish Mandlik

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Abstract

Background and aim

Kerala Ayurveda Ostoact Tablet (OAT) is a traditional Ayurvedic preparation that might use for the management of joint pain, bone pain and arthritis. The goal of present research was to evaluate the activity of OAT in improving postmenopausal osteoporosis (PMO) in ovariectomized (OVX) rats.

Experimental procedures

Female rats were ovariectomized bilaterally and distributed into 6 groups (n = 8) as Sham control (SC), Ovariectomy control (OVX), OVX with OAT (50 mg/kg, p.o.), OVX with OAT (100 mg/kg, p.o.) and OVX with OAT (150 mg/kg, p.o.) and Standard group (17β-estradiol, 30 μg/kg, s.c.). SC group rats went through a sham operation procedure. The animals were treated with an oral dose of OAT (50, 100 and 150 mg/kg) for 90 days. Body weight, tail skin temperature (TST) Serum hormones, bone turnover parameters, proinflammatory cytokines, bone physical parameters, histopathological analysis (uterus, vagina, and femur) and microcomputed tomography of the femur were measured on the 90th day of treatment. A new high performance thin layer chromatography (HPTLC) process has been established for the standardization of OAT.

Results and conclusion

OVX rats treated with OAT exhibited a significant decrease in TST, improved serum hormonal and lipid profile, bone turnover markers and pro-inflammatory cytokines, bone physical properties, increased bone density, and enhanced cytological and histological alterations with significant improvement in rat's body weight. In addition, OAT administration enhanced the weakening of trabecular bone microarchitecture triggered by OVX confirmed by microcomputed tomography (micro-CT). These findings imply that OAT may help treat osteoporosis in women brought on by menopause.

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喀拉拉阿育吠陀 Ostoact 片通过调节 RANKL/OPG 通路治疗卵巢切除大鼠模型的骨质疏松症

背景和目的喀拉拉邦阿育吠陀Ostoact片剂（OAT）是一种传统的阿育吠陀制剂，可用于治疗关节疼痛、骨痛和关节炎。本研究的目的是评估OAT在改善卵巢切除（OVX）大鼠绝经后骨质疏松症（PMO）中的活性。实验方法雌性大鼠双侧切除卵巢，分为6组（n = 8），分别为假对照组（SC）、卵巢切除对照组（OVX）、OAT组（50 mg/kg，口服）、OAT组（100 mg/kg，口服）、OAT组（150 mg/kg，口服）和标准组（17β-雌二醇，30 μg/kg，口服）。SC组大鼠进行假手术。动物分别口服50、100和150 mg/kg的OAT治疗90天。于治疗第90天测定大鼠体重、尾皮温度（TST）、血清激素、骨转换参数、促炎因子、骨物理参数、组织病理（子宫、阴道、股骨）及股骨微计算机断层扫描。建立了一种新的高效薄层色谱（HPTLC）方法，用于OAT的标准化。结果与结论经OAT处理的ovx大鼠TST显著降低，血清激素和脂质水平、骨转换标志物和促炎细胞因子改善，骨物理性质改善，骨密度增加，细胞学和组织学改变增强，体重显著改善。此外，经微计算机断层扫描（micro-CT）证实，OAT可增强OVX引发的骨小梁微结构弱化。这些发现表明，OAT可能有助于治疗女性因更年期引起的骨质疏松症。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.