Saccharomyces cerevisiae NX2320 inhibit Aspergillus carbonarius by cell membrane disruption and oxidative stress-mediated autophagy in in vitro and during grape storage

IF 3.7 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biological Control Pub Date : 2024-06-13 DOI:10.1016/j.biocontrol.2024.105559

Xixi Zhao , Liangfu Zhou , Xinglu Duan , Yanlin Liu

{"title":"Saccharomyces cerevisiae NX2320 inhibit Aspergillus carbonarius by cell membrane disruption and oxidative stress-mediated autophagy in in vitro and during grape storage","authors":"Xixi Zhao , Liangfu Zhou , Xinglu Duan , Yanlin Liu","doi":"10.1016/j.biocontrol.2024.105559","DOIUrl":null,"url":null,"abstract":"<div>Aspergillus carbonarius not only causes the decay of fresh fruit, but also produce the mycotoxin ochratoxin A. Biological control of pathogenic fungus by antagonistic microorganisms is a safe and effective method. However, information about the potential mechanism of antagonistic microorganisms to suppress A. carbonarius is limited. In this study, S. cerevisiae NX2320 inhibited the growth of A. carbonarius in PDA media and during grape storage. 2-phenylethanol (2-PE) produced by S. cerevisiae NX2320 may be the key substance for its antifungal mechanism. The result indicated that S. cerevisiae NX2320 and 2-PE inhibited OTA production, break the redox steady state, destroy membrane integrity, cause cell autophagy and control the production of mycotoxins. The results indicated that S. cerevisiae must be further investigated for potential application to control fungal and mycotoxin problems during grape storage.</div>","PeriodicalId":8880,"journal":{"name":"Biological Control","volume":"195 ","pages":"Article 105559"},"PeriodicalIF":3.7000,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1049964424001245/pdfft?md5=21c6b2bb9d546b18e08b5bf8869f671f&pid=1-s2.0-S1049964424001245-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Control","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1049964424001245","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aspergillus carbonarius not only causes the decay of fresh fruit, but also produce the mycotoxin ochratoxin A. Biological control of pathogenic fungus by antagonistic microorganisms is a safe and effective method. However, information about the potential mechanism of antagonistic microorganisms to suppress A. carbonarius is limited. In this study, S. cerevisiae NX2320 inhibited the growth of A. carbonarius in PDA media and during grape storage. 2-phenylethanol (2-PE) produced by S. cerevisiae NX2320 may be the key substance for its antifungal mechanism. The result indicated that S. cerevisiae NX2320 and 2-PE inhibited OTA production, break the redox steady state, destroy membrane integrity, cause cell autophagy and control the production of mycotoxins. The results indicated that S. cerevisiae must be further investigated for potential application to control fungal and mycotoxin problems during grape storage.

查看原文本刊更多论文

酿酒酵母 NX2320 在体外和葡萄贮藏期间通过细胞膜破坏和氧化应激介导的自噬抑制碳酸曲霉菌

碳曲霉不仅会导致新鲜水果腐烂，还会产生霉菌毒素赭曲霉毒素 A。然而，有关拮抗微生物抑制碳酸菌的潜在机制的信息还很有限。本研究中，S. cerevisiae NX2320 在 PDA 培养基中和葡萄贮藏期间抑制了 A. carbonarius 的生长。S. cerevisiae NX2320 产生的 2-苯基乙醇（2-PE）可能是其抗真菌机制的关键物质。结果表明，葡萄孢菌 NX2320 和 2-PE 可抑制 OTA 的产生，打破氧化还原稳态，破坏膜完整性，导致细胞自噬，并控制霉菌毒素的产生。研究结果表明，必须进一步研究 S. cerevisiae 的潜在应用，以控制葡萄贮藏过程中的真菌和霉菌毒素问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biological Control 生物-昆虫学

CiteScore

7.40

自引率

7.10%

发文量

220

审稿时长

63 days

期刊介绍： Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.