Performance of a point-of-care test for the detection of anti-Leishmania infantum antibodies is associated with immunofluorescent antibody titer and clinical stage of leishmaniosis in dogs from endemic regions

IF 1.4 Q3 PARASITOLOGY
Kristen Davenport , Joe Liu , Juliana Sarquis , Melissa Beall , Ana Montoya , Jan Drexel , Tori Denis , Ryan Toste , Donato Traversa , Guadalupe Miró
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引用次数: 0

Abstract

Canine leishmaniosis (CanL) is caused by the protozoal parasite Leishmania infantum, which is transmitted by sand flies in warm climates across the world. Because dogs are considered a primary domestic reservoir for the parasite that causes leishmaniosis in humans, it is important from a One Health perspective that CanL be properly managed. In endemic regions, CanL is a common differential diagnosis in sick dogs because the clinical signs and clinicopathological disorders of the disease are non-specific, variable, and may overlap those of other common conditions. Diagnosis is based on the presence of compatible clinical signs, laboratory abnormalities, and confirmation by serological and parasitological evidence of infection. Here, we describe the performance of a point-of-care (POC) immunoassay that uses recombinant antigens to detect canine anti- L. infantum antibodies in a convenience sample set from a diagnostic laboratory, a group of canine patients with clinical staging, and in apparently healthy dogs from endemic areas. An immunofluorescence antibody test (IFAT) was used as the semiquantitative reference method. In the convenience sample set with high IFAT titers (≥ 1:800), the POC immunoassay demonstrated perfect agreement with IFAT (100%; 90/90). Using samples from dogs staged as either LeishVet Stage 2 or 3 or LeishVet Stage 1, positive agreement of the POC immunoassay with the IFAT was 98.8% (82/83) and 83.8% (31/37), respectively. The negative agreement with IFAT was 98.9% (272/275) in apparently healthy dogs from endemic areas of Greece and Italy. Since the performance of the POC immunoassay was associated with IFAT titer and clinical stage of CanL, the test may help veterinarians when determining if CanL is likely responsible for a patient's clinical picture or when evaluating an apparently healthy patient prior to vaccination.

用于检测抗婴儿利什曼病抗体的护理点检验的性能与免疫荧光抗体滴度和利什曼病流行地区犬只的临床阶段有关
犬利什曼病(CanL)是由幼年利什曼原虫引起的,在全球温暖的气候条件下,幼年利什曼原虫由沙蝇传播。由于狗被认为是导致人类利什曼病的寄生虫的主要家畜库,因此从 "一体健康 "的角度来看,妥善管理犬利什曼病非常重要。在地方病流行地区,犬利什曼病是病犬的常见鉴别诊断,因为该病的临床症状和临床病理紊乱是非特异性的、多变的,并且可能与其他常见疾病重叠。诊断的依据是临床症状、实验室异常以及感染的血清学和寄生虫学证据。在这里,我们介绍了一种使用重组抗原的护理点(POC)免疫测定的性能,该方法可在诊断实验室的方便样本集、一组临床分期的犬病患者以及来自流行地区的明显健康的犬中检测犬抗幼犬病毒抗体。免疫荧光抗体测试(IFAT)被用作半定量参考方法。在 IFAT 滴度较高(≥ 1:800)的方便样本组中,POC 免疫测定与 IFAT 完全一致(100%;90/90)。使用分期为 LeishVet 2 或 3 期或 LeishVet 1 期的犬样本,POC 免疫测定与 IFAT 的阳性一致率分别为 98.8%(82/83)和 83.8%(31/37)。在希腊和意大利流行区的明显健康的狗身上,POC 免疫测定与 IFAT 的阴性吻合率为 98.9%(272/275)。由于 POC 免疫测定的性能与 IFAT 滴度和 CanL 的临床阶段有关,因此该检测可帮助兽医确定 CanL 是否可能导致患者的临床症状,或在接种疫苗前对表面健康的患者进行评估。
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来源期刊
CiteScore
2.90
自引率
7.10%
发文量
126
审稿时长
97 days
期刊介绍: Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).
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