Antibacterial activity of microwave synthesized hydroxyapatite against cariogenic bacteria: A preliminary study

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Saudi Dental Journal Pub Date : 2024-08-01 DOI:10.1016/j.sdentj.2024.06.004

{"title":"Antibacterial activity of microwave synthesized hydroxyapatite against cariogenic bacteria: A preliminary study","authors":"","doi":"10.1016/j.sdentj.2024.06.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>The effects of hydroxyapatite (HA) on oral bacteria and biofilm remains inconclusive, with conflicting results. Studies assessing its effect against caries-causing bacteria are limited.</p></div><div><h3>Objective</h3><p>This study aimed to explore the antibacterial activity of HA synthesized using microwave against two of the most common cariogenic bacteria, <em>Streptococcus mutans (S. mutans)</em> and <em>Streptococcus sobrinus (S. sobrinus).</em></p></div><div><h3>Methods</h3><p>HA was chemically synthesized using a microwave. To verify the existence of the crystalline phase and the calcium and phosphate content, X-ray diffraction (XRD) and energy-dispersive X-ray (EDX) analysis were employed, respectively. Reduction in bacterial growth was used to assess the antibacterial effects of 10 %, 20 %, and 30 % HA against the tested bacteria.</p></div><div><h3>Results</h3><p>The presence of the hydroxyapatite crystallite phase was verified using XRD, while EDX revealed the Calcium to Phosphorus (Ca/P) ratio to be 1.6. In response to the 10 %, 20 %, and 30 % HA, <em>S. mutans</em> were reduced by 14.5 %, 15.6 %, and 23.4 %, whereas <em>S. sobrinus</em> decreased by 17.1 %, 60.8 %, and 98.6 %, respectively.</p></div><div><h3>Conclusion</h3><p>Microwave-synthesized HA could have antibacterial properties against caries-causing bacteria with different potencies depending on concentration and bacteria.</p></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S101390522400172X/pdfft?md5=1c288ca3fd339d73f60f6caab0c0bac7&pid=1-s2.0-S101390522400172X-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Saudi Dental Journal","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S101390522400172X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

The effects of hydroxyapatite (HA) on oral bacteria and biofilm remains inconclusive, with conflicting results. Studies assessing its effect against caries-causing bacteria are limited.

Objective

This study aimed to explore the antibacterial activity of HA synthesized using microwave against two of the most common cariogenic bacteria, Streptococcus mutans (S. mutans) and Streptococcus sobrinus (S. sobrinus).

Methods

HA was chemically synthesized using a microwave. To verify the existence of the crystalline phase and the calcium and phosphate content, X-ray diffraction (XRD) and energy-dispersive X-ray (EDX) analysis were employed, respectively. Reduction in bacterial growth was used to assess the antibacterial effects of 10 %, 20 %, and 30 % HA against the tested bacteria.

Results

The presence of the hydroxyapatite crystallite phase was verified using XRD, while EDX revealed the Calcium to Phosphorus (Ca/P) ratio to be 1.6. In response to the 10 %, 20 %, and 30 % HA, S. mutans were reduced by 14.5 %, 15.6 %, and 23.4 %, whereas S. sobrinus decreased by 17.1 %, 60.8 %, and 98.6 %, respectively.

Conclusion

Microwave-synthesized HA could have antibacterial properties against caries-causing bacteria with different potencies depending on concentration and bacteria.

查看原文本刊更多论文

微波合成羟基磷灰石对致癌细菌的抗菌活性：初步研究

引言羟基磷灰石（HA）对口腔细菌和生物膜的影响尚无定论，结果相互矛盾。本研究旨在探讨用微波合成的羟基磷灰石对两种最常见的致龋细菌--变异链球菌（S. mutans）和索布里诺链球菌（S. sobrinus）--的抗菌活性。为了验证结晶相的存在以及钙和磷酸盐的含量，分别采用了 X 射线衍射 (XRD) 和能量色散 X 射线 (EDX) 分析。结果使用 XRD 验证了羟基磷灰石结晶相的存在，而 EDX 则显示钙磷（Ca/P）比为 1.6。在 10 %、20 % 和 30 % HA 的作用下，突变杆状病毒分别减少了 14.5 %、15.6 % 和 23.4 %，而梭状芽孢杆菌则分别减少了 17.1 %、60.8 % 和 98.6 %。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Saudi Dental Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Saudi Dental Journal is an English language, peer-reviewed scholarly publication in the area of dentistry. Saudi Dental Journal publishes original research and reviews on, but not limited to: • dental disease • clinical trials • dental equipment • new and experimental techniques • epidemiology and oral health • restorative dentistry • periodontology • endodontology • prosthodontics • paediatric dentistry • orthodontics and dental education Saudi Dental Journal is the official publication of the Saudi Dental Society and is published by King Saud University in collaboration with Elsevier and is edited by an international group of eminent researchers.