Unfurling an improved method for visualizing mitotic chromosomes in ferns

IF 2.7 3区生物学 Q2 PLANT SCIENCES

Applications in Plant Sciences Pub Date : 2024-06-04 DOI:10.1002/aps3.11588

Rosa Ramirez-Castillo, Claudio Palma-Rojas, Pedro Jara Seguel, Amanda L. Grusz, Cristian Araya-Jaime

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引用次数: 0

Abstract

Premise

Cytotaxonomy employs chromosome visualization to study organismal relationships and evolution. Despite the critical value of cytogenetic data, cytotypes are lacking for many plant groups. Here, we present an improved approach for visualizing mitotic chromosomes in ferns, a key lineage of land plants, using the dividing cells of unfurling croziers (fiddleheads).

Methods and Results

Our modified mitotic chromosome preparation incorporates a brief pectinase–cellulase pretreatment, as well as colchicine fixation and the Feulgen reaction to improve the staining and separation of mitotic chromosomes. To demonstrate this easy and efficient assessment, we determined the sporophytic (2n) chromosome number for three fern species: Cheilanthes mollis (2n = 60), Cheilanthes hypoleuca (2n = 120), and Nephrolepis cordifolia (2n = 82).

Conclusions

The new method presented here improves visualizations of mitotic chromosomes from the dividing nuclei of young fern croziers. Fiddleheads are widely accessible in nature and in living collections worldwide, and this modified approach increases their suitability for fern cytotaxonomic studies.

Abstract Image

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展开蕨类植物有丝分裂染色体可视化的改进方法

细胞分类学利用染色体可视化来研究生物的关系和进化。尽管细胞遗传学数据具有重要价值，但许多植物类群都缺乏细胞型。我们改进的有丝分裂染色体制备方法包括简短的果胶酶-纤维素酶预处理、秋水仙碱固定和费尔根反应，以改善有丝分裂染色体的染色和分离。为了证明这种简便高效的评估方法，我们测定了三种蕨类植物的孢子体染色体数（2n）：本文介绍的新方法提高了从蕨类植物幼苗分裂的细胞核中观察有丝分裂染色体的效率。蕨菜在自然界和世界各地的活体收藏中广泛存在，这种改良方法提高了蕨菜细胞分类学研究的适用性。

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来源期刊

Applications in Plant Sciences PLANT SCIENCES-

CiteScore

7.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences. APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.