Pathophysiological changes and injury markers for acute lung injury from blunt impact in infant rabbits

Ke Wang, ZhenPeng Huang, JiaWei He, Ling-wang Kong, Mingwei Chen
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Abstract

Traffic accidents, particularly blunt impacts, cause serious injuries in children. We aimed to assess inflammatory and injury responses in infant rabbits subjected to acute lung injury resulting from blunt impact, with the goal of identifying potential circulatory injury markers.Forty 4-week-old infant rabbits were subjected to a right chest impact using a Hopkinson bar with 2,600 g. Computed tomography was employed to assess injury severity. Pathological changes were observed using hematoxylin and eosin staining in the control, 0, 24, and 72 h groups, post-injury. Immunohistochemistry was used to examine surfactant protein A (SP-A) changes in right lung tissues and upper main bronchi. Serum levels of interleukin-6 (IL-6), IL-8, and SP-A were measured using ELISA within 24 h post-injury in the control, 0 h, and 24 h groups.Following blunt injury, significant increases were observed in blood white blood cell count (F = 101.556, P < 0.01) and neutrophil percentage (F = 104.228, P < 0.01), which gradually decreased after 24 and 72 h. The lung wet/dry weight ratio indicated significant edema (F = 79.677, P < 0.01), corroborated by hematoxylin and eosin staining showing edema, exudation, and marked granulocyte infiltration in the control, 0 h, 24 h and 72 h groups. SP-A levels decreased rapidly at 0 h, and recovered between 24 and 72 h in the right lung tissues (F = 6.7, P < 0.05), left lung (F = 15.825, P < 0.05) and upper main bronchi (F = 59.552, P < 0.01). The ELISA results showed increasing trends for the control and 0 h groups, while decreasing trends were observed in 24 h group for IL-6 (F = 58.328, P < 0.01) and IL-8 (F = 41.802, P < 0.01). Conversely, SP-A exhibited a decreasing trend in the control and 0 h groups but increased in the serum of 24 h group (F = 52.629, P < 0.01).In cases of direct chest trauma in infant rabbits, particularly mild injuries without rib fractures. SP-A levels correlated with pathological changes across all groups and may serve as biomarkers for pediatric blunt lung impact.
钝器撞击造成幼兔急性肺损伤的病理生理学变化和损伤标志物
交通事故,尤其是钝器撞击,会对儿童造成严重伤害。我们的目的是评估因钝性撞击导致急性肺损伤的幼兔的炎症和损伤反应,以确定潜在的循环损伤标志物。采用计算机断层扫描评估损伤严重程度。使用苏木精和伊红染色法观察对照组、受伤后 0、24 和 72 小时组的病理变化。免疫组化技术用于检测右肺组织和上主支气管中表面活性蛋白 A(SP-A)的变化。钝挫伤后,血白细胞计数(F = 101.556,P < 0.01)和中性粒细胞百分比(F = 104.228,P < 0.01)显著增加,24 和 72 h 后逐渐减少。对照组、0 小时组、24 小时组和 72 小时组的肺干湿重比显示肺明显水肿(F = 79.677,P < 0.01),苏木精和伊红染色显示水肿、渗出和明显的粒细胞浸润也证实了这一点。在右肺组织(F = 6.7,P < 0.05)、左肺(F = 15.825,P < 0.05)和上主支气管(F = 59.552,P < 0.01)中,SP-A 水平在 0 h 迅速下降,并在 24 和 72 h 之间恢复。ELISA结果显示,对照组和0小时组呈上升趋势,而24小时组的IL-6(F = 58.328,P < 0.01)和IL-8(F = 41.802,P < 0.01)呈下降趋势。相反,SP-A 在对照组和 0 h 组呈下降趋势,但在 24 h 组血清中呈上升趋势(F = 52.629,P < 0.01)。SP-A水平与各组病理变化相关,可作为小儿钝性肺损伤的生物标记物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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