Monitoring estuarine fish communities – environmental DNA (eDNA) metabarcoding as a complement to beach seining

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-10 DOI:10.1139/cjfas-2023-0227

Mark Saunders, R. Steeves, Leah P. Macintyre, Kyle M. Knysh, Michael Coffin, M. Boudreau, Christina C. Pater, M R van den Heuvel, S. Courtenay

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引用次数: 0

Abstract

Environmental DNA (eDNA) metabarcoding offers advantages over physical capture for identifying and quantifying animals in monitoring programs. In this study, the fish community was sampled at three stations (inner, middle, and outer estuary) in three estuaries in August 2020, and four estuaries in June and August 2021 (Prince Edward Island, Canada) using both beach seining and eDNA metabarcoding. Two 12S primer sets, 12S-160 and 12S-248F, with different amplicon lengths, yielded similar results. eDNA metabarcoding consistently detected species captured by 186 co-located beach seines and revealed additional species. It also detected monthly (June-August), interannual (2020-2021), and spatial shifts in the fish community, distinguishing stations separated by as little as 0.4 km. Positive correlations existed between eDNA metabarcoding species reads and beach seining captures. These findings suggest eDNA metabarcoding complements physical capture methods for characterising nearshore fish communities in Prince Edward Island’s estuaries. While eDNA techniques lack certain population parameter information provided by physical methods, such as size, sex, and age structure, they offer a more comprehensive diversity assessment and presence-abundance insights, especially in inaccessible environments.

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监测河口鱼类群落--环境 DNA (eDNA) 代谢标定是对海滩围网的补充

环境 DNA (eDNA) 代谢编码在监测项目中识别和量化动物方面比物理捕获更具优势。在这项研究中，利用海滩围网和 eDNA 元标定技术，于 2020 年 8 月在三个河口的三个站点（内河口、中河口和外河口）以及 2021 年 6 月和 8 月在四个河口（加拿大爱德华王子岛）对鱼类群落进行了采样。两种 12S 引物组（12S-160 和 12S-248F，具有不同的扩增子长度）产生了相似的结果。eDNA 代谢编码可持续检测到 186 个共定位海滩围网捕获的物种，并发现更多物种。它还能检测到鱼类群落的月度（6 月至 8 月）、年际（2020 年至 2021 年）和空间变化，并将相距仅 0.4 公里的站点区分开来。eDNA 代谢标码物种读数与海滩围网捕获量之间存在正相关。这些研究结果表明，在描述爱德华王子岛河口近岸鱼类群落特征时，eDNA 代谢标定是对物理捕获方法的补充。虽然 eDNA 技术缺乏物理方法提供的某些种群参数信息，如大小、性别和年龄结构，但它们提供了更全面的多样性评估和存在-丰度洞察力，尤其是在难以接近的环境中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.