Enhancing Bovine Embryo Development In Vitro Using Oil-in-Water Nanoemulsions as Specific Carriers for Essential Lipids

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioTech Pub Date : 2024-06-11 DOI:10.3390/biotech13020019
Daniel Enrique López Angulo, R. Lourenço, A. Bridi, M. A. Chaves, J. D. da Silveira, P. J. Sobral
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Abstract

Worldwide meat consumption and production have nearly quintupled in the last 60 years. In this context, research and the application of new technologies related to animal reproduction have evolved in an accelerated way. The objective of the present study was to apply nanoemulsions (NEs) as carriers of lipids to feed bovine embryos in culture media and verify their impact on the development of embryos produced in vitro. The NEs were characterized by particle size, polydispersity, size distribution, physical stability, morphology using atomic force microscopy (AFM), surface tension, density, pH, and rheological behavior. The NEs were prepared by the emulsification/evaporation technique. A central composite rotatable design (CCRD) was used to optimize the NE fabrication parameters. The three optimized formulations used in the embryo application showed an emulsion stability index (ESI) between 0.046 and 0.086, which reflects high stability. The mean droplet diameter analyzed by laser diffraction was approximately 70–80 nm, suggesting a possible transit across the embryonic zona pellucida with pores of an average 90 nm in diameter. AFM images clearly confirm the morphology of spherical droplets with a mean droplet diameter of less than 100 nm. The optimized formulations added during the higher embryonic genome activation phase in bovine embryos enhanced early embryonic development.
利用水包油型纳米乳液作为必需脂质的特异性载体促进牛胚胎体外发育
在过去的 60 年中,全球肉类消费和生产几乎翻了五番。在这种情况下,与动物繁殖相关的新技术的研究和应用也在加速发展。本研究的目的是应用纳米乳液(NEs)作为脂质载体,在培养基中喂养牛胚胎,并验证其对体外生产的胚胎发育的影响。纳米乳液的特征包括粒度、多分散性、粒度分布、物理稳定性、使用原子力显微镜(AFM)观察的形态、表面张力、密度、pH 值和流变行为。NEs 采用乳化/蒸发技术制备。采用中心复合可旋转设计(CCRD)来优化 NE 的制造参数。胚胎应用中使用的三种优化配方的乳液稳定性指数(ESI)介于 0.046 和 0.086 之间,体现了较高的稳定性。通过激光衍射分析,液滴的平均直径约为 70-80 nm,这表明液滴有可能穿过胚胎透明带,其孔隙的平均直径为 90 nm。原子力显微镜图像清楚地证实了平均液滴直径小于 100 纳米的球形液滴形态。在牛胚胎较高的胚胎基因组激活阶段添加的优化配方可促进早期胚胎发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BioTech
BioTech Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
3.70
自引率
0.00%
发文量
51
审稿时长
11 weeks
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