The miRNA Contribution in Adipocyte Maturation

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Non-Coding RNA Pub Date : 2024-06-12 DOI:10.3390/ncrna10030035

A. Giammona, S. Di Franco, Alessia Lo Dico, Giorgio Stassi

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引用次数: 0

Abstract

Mesenchymal stem cells, due to their multipotent ability, are considered one of the best candidates to be used in regenerative medicine. To date, the most used source is represented by the bone marrow, despite the limited number of cells and the painful/invasive procedure for collection. Therefore, the scientific community has investigated many alternative sources for the collection of mesenchymal stem cells, with the adipose tissue representing the best option, given the abundance of mesenchymal stem cells and the easy access. Although adipose mesenchymal stem cells have recently been investigated for their multipotency, the molecular mechanisms underlying their adipogenic potential are still unclear. In this scenario, this communication is aimed at defining the role of miRNAs in adipogenic potential of adipose-derived mesenchymal stem cells via real-time PCR. Even if preliminary, our data show that cell culture conditions affect the expression of specific miRNA involved in the adipogenic potential of mesenchymal stem cells. The in vitro/in vivo validation of these results could pave the way for novel therapeutic strategies in the field of regenerative medicine. In conclusion, our research highlights how specific cell culture conditions can modulate the adipogenic potential of adipose mesenchymal stem cells through the regulation of specific miRNAs.

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miRNA 在脂肪细胞成熟过程中的作用

间充质干细胞具有多能性，被认为是再生医学的最佳候选细胞之一。迄今为止，使用最多的来源是骨髓，尽管骨髓中的细胞数量有限，而且采集过程痛苦/侵入性强。因此，科学界研究了许多收集间充质干细胞的替代来源，其中脂肪组织是最佳选择，因为它含有丰富的间充质干细胞，而且容易获取。尽管最近对脂肪间充质干细胞的多潜能性进行了研究，但其脂肪形成潜能的分子机制仍不清楚。在这种情况下，这篇通讯旨在通过实时 PCR 确定 miRNA 在脂肪间充质干细胞成脂潜能中的作用。尽管是初步研究，但我们的数据表明，细胞培养条件会影响参与间充质干细胞成脂潜能的特定 miRNA 的表达。这些结果的体外/体内验证可为再生医学领域的新型治疗策略铺平道路。总之，我们的研究强调了特定的细胞培养条件如何通过调控特定的miRNA来调节脂肪间充质干细胞的成脂潜能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.