Yukun Liu, Xingliang Xiang, Zhaoxiang Zeng, Meiling Guo, Yiping Li, Zecai Zhan, Rongzeng Huang, Min Zhao, Shuna Jin, Chengwu Song
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引用次数: 0
Abstract
Fuzhuan brick tea (FBT), a post-fermented tea, has been demonstrated to have an antidiabetic effect. However, evidence of its hypoglycemic effect via gut microbiota and intestinal metabolites has been limited. This work aimed to reveal the potential mechanism of FBT against type 2 diabetes mellitus (T2DM) mice by regulating gut flora and short-chain fatty acids (SCFAs). Thus, we established a T2DM mice model induced by streptozotocin and a high-fat diet, and the 16S rDNA gene sequencing analysed gut microbiota changes, while GC/MS detected SCFAs levels in colonic contents. As a result, after being treated with FBT, the biochemical index of mice revealed significant improvement and the levels of colonic SCFAs were enhanced. Through correlation analysis, the key gut microbiota for the hypoglycemic effect of FBT were identified including Cyanobacteria, Alloprevotella, Erysipelatoclostridium, etc. Additionally, the antidiabetic actions of FBT were mediated by the SCFAs-GPR41/43-GLP-1 pathway and alleviating inflammation related to the increased gut barrier with the tight junction proteins (Claudin-1, Occludin and ZO-1). To sum up, our study provides insights into the possible mechanism of FBT in T2DM and might promote the application of FBT as a potential hypoglycemic agent in the future.
期刊介绍:
The International Journal of Food Science & Technology (IJFST) is published for the Institute of Food Science and Technology, the IFST. This authoritative and well-established journal publishes in a wide range of subjects, ranging from pure research in the various sciences associated with food to practical experiments designed to improve technical processes. Subjects covered range from raw material composition to consumer acceptance, from physical properties to food engineering practices, and from quality assurance and safety to storage, distribution, marketing and use. While the main aim of the Journal is to provide a forum for papers describing the results of original research, review articles are also welcomed.