Genome Sequencing Analysis of a Rare Case of Blood Infection Caused by Flavonifractor plautii.

IF 1 Q3 MEDICINE, GENERAL & INTERNAL
Xingying Chen, Wei Bi, Xinyi Ruan, Limin Jin, Nenghua Zhang
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引用次数: 0

Abstract

BACKGROUND Flavonifractor plautii belongs to the clostridium family, which can lead to local infections as well as the bloodstream infections. Flavonifractor plautii caused infection is rarely few in the clinic. To understand better Flavonifractor plautii, we investigated the drug sensitivity and perform genome sequencing of Flavonifractor plautii isolated from blood samples in China and explored the drug resistance and pathogenic mechanism of the bacteria. CASE REPORT The Epsilometer test method was used to detect the sensitivity of flavonoid bacteria to antimicrobial agents. PacBio sequencing technology was employed to sequence the whole genome of Flavonifractor plautii, and gene prediction and functional annotation were also analyzed. Flavonifractor plautii displayed sensitivity to most drugs but resistance to fluoroquinolones and tetracycline, potentially mediated by tet (W/N/W). The total genome size of Flavonifractor plautii was 4,573,303 bp, and the GC content was 59.78%. Genome prediction identified 4,506 open reading frames, including 9 ribosomal RNAs and 66 transfer RNAs. It was detected that the main virulence factor-coding genes of the bacteria were the capsule, polar flagella and FbpABC, which may be associated with bacterial movement, adhesion, and biofilm formation. CONCLUSIONS The results of whole-genome sequencing could provide relevant information about the drug resistance mechanism and pathogenic mechanism of bacteria and offer a basis for clinical diagnosis and treatment.

黄癣菌引起的罕见血液感染病例的基因组测序分析
背景 黄癣菌属于梭状芽孢杆菌科,可导致局部感染和血液感染。在临床上,黄腐菌引起的感染很少见。为了更好地了解黄癣菌,我们对从中国血液样本中分离出的黄癣菌进行了药物敏感性研究和基因组测序,并探讨了该细菌的耐药性和致病机制。病例报告 采用Epsilometer测试法检测黄杆菌对抗菌药物的敏感性。采用 PacBio 测序技术对黄杆菌进行了全基因组测序,并进行了基因预测和功能注释分析。Flavonifractor plautii对大多数药物敏感,但对氟喹诺酮类药物和四环素类药物耐药,这可能是由tet(W/N/W)介导的。Flavonifractor plautii的基因组总大小为4,573,303 bp,GC含量为59.78%。基因组预测确定了 4,506 个开放阅读框,包括 9 个核糖体 RNA 和 66 个转移 RNA。检测发现,细菌的主要毒力因子编码基因为胶囊、极鞭毛和 FbpABC,这些基因可能与细菌的移动、粘附和生物膜的形成有关。结论 全基因组测序结果可提供细菌耐药机制和致病机制的相关信息,为临床诊断和治疗提供依据。
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来源期刊
American Journal of Case Reports
American Journal of Case Reports Medicine-Medicine (all)
CiteScore
1.80
自引率
0.00%
发文量
599
期刊介绍: American Journal of Case Reports is an international, peer-reviewed scientific journal that publishes single and series case reports in all medical fields. American Journal of Case Reports is issued on a continuous basis as a primary electronic journal. Print copies of a single article or a set of articles can be ordered on demand.
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