Strategies for the Isolation and Identification of Gastric Cancer Stem Cells.

IF 3.8 3区医学 Q2 CELL & TISSUE ENGINEERING

Stem Cells International Pub Date : 2024-06-06 eCollection Date: 2024-01-01 DOI:10.1155/2024/5553852

Jianhua Wang, Jie Qu, Qiang Hou, Xueping Huo, Xiangrong Zhao, Le Chang, Cuixiang Xu

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引用次数: 0

Abstract

Gastric cancer stem cells (GCSCs) originate from both gastric adult stem cells and bone marrow cells and are conspicuously present within the histological milieu of gastric cancer tissue. GCSCs play pivotal and multifaceted roles in the initiation, progression, and recurrence of gastric cancer. Hence, the characterization of GCSCs not only facilitates precise target identification for prospective therapeutic interventions in gastric cancer but also has significant implications for targeted therapy and the prognosis of gastric cancer. The prevailing techniques for GCSC purification involve their isolation using surface-specific cell markers, such as those identified by flow cytometry and immunomagnetic bead sorting techniques. In addition, in vitro culture and side-population cell sorting are integral methods in this context. This review discusses the surface biomarkers, isolation techniques, and identification methods of GCSCs, as well as their role in the treatment of gastric cancer.

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胃癌干细胞的分离和鉴定策略

胃癌干细胞（GCSCs）来源于胃成体干细胞和骨髓细胞，明显存在于胃癌组织的组织学环境中。胃癌干细胞在胃癌的发生、发展和复发过程中发挥着多方面的关键作用。因此，GCSCs 的特征描述不仅有助于胃癌前瞻性治疗干预的精确靶点识别，而且对胃癌的靶向治疗和预后具有重要意义。目前流行的胃癌细胞间充质干细胞纯化技术包括利用表面特异性细胞标记物（如流式细胞术和免疫磁珠分选技术所识别的细胞标记物）进行分离。此外，体外培养和侧群细胞分选也是这方面不可或缺的方法。本综述将讨论胃癌细胞的表面生物标记物、分离技术和鉴定方法，以及它们在胃癌治疗中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem Cells International CELL & TISSUE ENGINEERING-

CiteScore

8.10

自引率

2.30%

发文量

188

审稿时长

18 weeks

期刊介绍： Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials. Topics covered include, but are not limited to: embryonic stem cells; induced pluripotent stem cells; tissue-specific stem cells; stem cell differentiation; genetics and epigenetics; cancer stem cells; stem cell technologies; ethical, legal, and social issues.