Effects of hormone sources on developmental competence of oocytes by ovum pickup in Japanese black cattle

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2024-06-13 DOI:10.1016/j.anireprosci.2024.107533

Zhihui Liu , Yanling Chen , Mengjia Huang , Yinyan Du , Guangyong Xu , Zhentian Liu , Meijie Zhang , Giorgio Antonio Presicce , Xuesong Xing , Fuliang Du

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Abstract

Japanese Black (Wagyu) cattle donors were primed with different protocols and sources of follicle-stimulating hormone (FSH) for successive ovum pickup (OPU) and embryo development after in vitro fertilization (IVF). Following OPU, retrieved cumulus oocyte complexes (COCs) were subjected to IVF, and resulting blastocysts were transferred into recipients to evaluate implantation capability. Experiment 1: The best blastocyst development (45.3 %) and embryo yields (5.0/donor/OPU) were found with oocytes retrieved from donors treated with FSH (STIMUFOL®, Belgium) at a dosage of 150 IU per donor, compared to two others commercial FSH sources. Experiment 2: There were no differences in embryo development or yield with STIMUFOL FSH (total FSH 150 IU/donor) at a priming duration of either 60-h (Regime 1, six FSH injections) or 36-h (Regime 2, four FSH injections). Experiment 3: Compacted COCs required 22–26-h maturation in vitro (IVM) before IVF for optimal blastocyst development (36.1–41.1 %); however, short (18-h) and prolonged (30-h) IVM duration resulted in lower embryonic development. In contrast, expanded COCs resulted in inferior blastocyst development compared to compacted COCs. Immunofluorescence microscopy revealed that the ratio of 89.8 % cumulus compacted COCs were at the germinal vesicle (pachytene) phase while 98.9 % cumulus expanded COCs went through spontaneous meiosis from meiotic metaphase I, anaphase I, telophase I to metaphase II upon OPU retrieval (P<0.05). Pregnancy rates were not different among three FSH sources or different FSH treatments as long as embryos reached the blastocyst stage. Our study found that different sources of FSH used for Wagyu donor priming prior to OPU resulted in differential embryo development potentials, but those embryos that reached out to blastocysts had a competent implantation ability.

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激素来源对日本黑牛卵母细胞发育能力的影响

日本黑毛和牛（Wagyu）供体在体外受精（IVF）后使用不同方案和不同来源的卵泡刺激素（FSH）进行连续取卵（OPU）和胚胎发育。取卵（OPU）后，对取回的积卵母细胞复合体（COC）进行体外受精，并将获得的囊胚移植到受体中，以评估植入能力。实验 1：与其他两种商业 FSH 来源相比，用 FSH（STIMUFOL®，比利时）处理供体的卵母细胞的囊胚发育（45.3%）和胚胎产量（5.0/供体/OPU）最好，剂量为每个供体 150 IU。实验 2：使用 STIMUFOL FSH（FSH 总量为 150 IU/供体），启动时间为 60 小时（方案 1，注射 6 次 FSH）或 36 小时（方案 2，注射 4 次 FSH），胚胎发育或产量没有差异。实验 3：压实的 COC 在体外受精前需要 22-26 小时的体外成熟期（IVM），以获得最佳的囊胚发育（36.1-41.1%）；然而，较短（18 小时）和较长（30 小时）的体外成熟期导致胚胎发育较低。相比之下，膨大的 COCs 的囊胚发育要比压缩的 COCs 差。免疫荧光显微镜检查显示，89.8%的积聚压实的COCs处于生殖泡（pachytene）期，而98.9%的积聚膨大的COCs在取回OPU时经历了自发减数分裂，从减数分裂的分裂期I、无核期I、端期I到分裂期II（P<0.05）。只要胚胎达到囊胚期，三种FSH来源或不同FSH处理的妊娠率没有差异。我们的研究发现，不同来源的 FSH 在 OPU 前用于和牛供体引物会导致不同的胚胎发育潜能，但达到囊胚期的胚胎具有合格的植入能力。

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.