Exploration of Anethum graveolens diversity from North Western Himalayan

Abstract

Anethum graveolens L., an aromatic plant grown during winter season belongs to the family Apiaceae. The leaves and seeds of the plants are widely consumed in India. The seeds of the plants are rich in aroma, due to the richness of essential oils whereas the leaves hold immense potential due to their antioxidant and heavy metal stress alleviation properties. However, the genetic diversity of A. graveolens from North Western Himalayan has not been explored so far. Therefore, the genetic diversity of A. graveolens was investigated using 33 random amplified polymorphic (RAPD) and 27 inter sequence specific region (ISSR) markers. Out of the 33 random primers, only 4 RAPD markers resulted in PCR amplification whereas 18 ISSR markers resulted in reproducible PCR amplification. Out of these ISSR markers, 11 markers accounted for 75–100 % of the polymorphism across all the accessions. Additionally, protein profiling was also used to investigate the diversity of A. graveolens landraces. Overall, the cluster analysis using Neighbor joining (N-J) and unweighted pair group method with arithmetic mean (UPGMA) method followed by principal component analysis (PCA) revealed that the accessions of A. graveolens irrespective to their geographic distribution shared the common group. Furthermore, the antioxidant potential of the local landraces was also investigated based on the free radical scavenging potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The present current study on exploration of A. graveolens diversity can be helpful in developing conservation strategies of its local germplasm prevalent in North Western Himalayan region.