Characterization of CD83 homologs differently expressed during monocytes differentiation in ginbuna crucian carp, Carassius auratus langsdorfii.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Trang Thu Tran, Harsha Prakash, Takahiro Nagasawa, Miki Nakao, Tomonori Somamoto
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Abstract

CD83 is a costimulatory molecule of antigen-presenting cells (APCs) that plays an important role in eliciting adaptive responses. It is also a well-known surface protein on mature dendritic cells (DCs). Furthermore, monocytes have been reported to differentiate into macrophages and monocyte-derived dendritic cells, which play an important role in innate immunity. CD83 expression affects the activation and maturation of DCs and stimulates cell-mediated immune responses. This study aims to reveal the CD83 expression during monocyte differentiation in teleosts, and the CD83 homologs evolutionary relationship. This study found two distinct CD83 homologs (GbCD83 and GbCD83-L) in ginbuna crucian carp (Gb) and investigated the evolutionary relationship among GbCD83 homologs and other vertebrates and the gene and protein expression levels of the homologs during 4 days of monocyte culture. The phylogenetic tree showed that the two GbCD83 homologs are classified into two distinct branches. Interestingly, only ostariophysians (Gb, common carp, rohu, fathead minnow and channel catfish), but not neoteleosts, mammals, and others, have two CD83 homologs. Morphological observation and colony-stimulating factor-1 receptor (CSF-1R), CD83, CD80/86, and CCR7 gene expressions illustrated that there is a differentiation of monocytes isolated from peripheral blood leukocytes after 4 days. Specifically, gene expression and immunocytochemistry revealed that GbCD83 is mainly expressed on monocytes at the early stage of cell culture, whereas GbCD83-L is expressed in the latter stage. These findings provided the first evidence of differential expression of CD83 homologs during monocytes differentiation in teleost.

鲫鱼单核细胞分化过程中不同表达的 CD83 同源物的特征。
CD83 是抗原递呈细胞(APC)的一种成本刺激分子,在诱发适应性反应中发挥着重要作用。它也是成熟树突状细胞(DC)上的一种众所周知的表面蛋白。此外,有报道称单核细胞可分化为巨噬细胞和单核细胞衍生树突状细胞,它们在先天免疫中发挥着重要作用。CD83 的表达会影响 DC 的活化和成熟,并刺激细胞介导的免疫反应。本研究旨在揭示长尾目动物单核细胞分化过程中 CD83 的表达以及 CD83 同源物的进化关系。本研究在银鲫中发现了两种不同的CD83同源物(GbCD83和GbCD83-L),并研究了GbCD83同源物与其他脊椎动物的进化关系,以及同源物在4天单核细胞培养过程中的基因和蛋白表达水平。系统发生树显示,两个GbCD83同源物被分为两个不同的分支。有趣的是,只有食肉目动物(Gb、鲤鱼、鲫鱼、胖头鱼和鲴鱼)有两个 CD83 同源物,而新食肉目动物、哺乳动物和其他动物则没有。形态学观察和集落刺激因子-1 受体(CSF-1R)、CD83、CD80/86 和 CCR7 基因表达表明,从外周血白细胞中分离的单核细胞在 4 天后出现了分化。具体来说,基因表达和免疫细胞化学显示,GbCD83 主要在细胞培养早期在单核细胞上表达,而 GbCD83-L 则在后期表达。这些发现首次证明了CD83同源物在远洋鱼类单核细胞分化过程中的差异表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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