Characterization, anti-microbial, anti-oxidant and growth promoting effects of biogenically synthesized silver nanoparticles derived from Dicliptera bupleuroides Nees

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2024-06-15 DOI:10.1002/jemt.24630

Memoona Samad, Wasim Akhtar, Arooj Muneer, Iram Fatima, Muhammad Manzoor, Syed Waseem Gillani, Fazal Ur Rehman, Khawar Majeed, Baber Ali, Rizwan Sarwar

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引用次数: 0

Abstract

One of the most important areas of nanotechnology is the use of nanoparticles (NPs) for a variety of environmental and biological applications, with silver nanoparticles (Ag-NPs) gaining a lot attention due to their distinct properties. The current study deals with the synthesis of Ag-NPs from Dicliptera bupleuroides Nees leaf extract and to determine their antioxidant, antimicrobial potential and effects on wheat seed germination and growth. UV–Visible spectrum revealed a prominent absorption peak at 442 nm, elucidating the conformation of the Ag-NPs synthesis. Scanning electron microscopy (SEM) showed distinctive triangular, pyramidal, and irregular shape. X-ray diffraction (XRD) demonstrated their crystalline nature, with average crystallite size of the Ag-NPs measured at 20.52 nm. Fourier-transform infrared spectroscopy (FT-IR) further confirmed the presence of functional groups such as Phenols (O-H stretch), transition metal carbonyls N-H, ≡C-H, C ≡ N, C ≡ C, C-Cl, C-Br and O-H bonds on the surface Ag-NPs. The antibacterial activity of the Ag-NPs was most pronounced against Bacillus subtilis, with a zone of inhibition (ZOI) measuring 11 mm ± 0.57 at a concentration of 1000 μg/mL (45% inhibition). Likewise, Ag-NPs exhibited highest antioxidant potential (73.2%) at 100 μg/mL compared with standard (ascorbic acid) which showed (76%) at the same concentration. Furthermore, the effect of D. bupleuroides mediated Ag-NPs on wheat seeds growth and germination was recorded maximum at high concentrations (200-300 ppm). In conclusion, D. bupleuroides mediated Ag-NPs showed safe, cost effective and environmentally friendly synthesis which can be used as an antibacterial and antioxidant agent as well as for enhancing the growth and seed germination of crop seeds globally.

Research Highlights

Nanotechnology is the study of nanoparticles for biological and environmental applications.
Ag-NPs among other NPs have received broad attention because of their unique properties.
D. bupleuroides Ag-NPs: 45% antibacterial, 73.2% antioxidant, enhance wheat germination.
D. bupleuroides-mediated Ag-NPs are both cost-effective and environmentally beneficial.

查看原文本刊更多论文

从 Dicliptera bupleuroides Nees 提取的生物合成银纳米粒子的特性、抗微生物、抗氧化和促进生长作用。

纳米技术最重要的领域之一是将纳米粒子（NPs）用于各种环境和生物应用，其中银纳米粒子（Ag-NPs）因其独特的性质而备受关注。目前的研究涉及从 Dicliptera bupleuroides Nees 叶提取物中合成 Ag-NPs，并确定其抗氧化、抗菌潜力以及对小麦种子发芽和生长的影响。紫外-可见光谱显示，在 442 纳米处有一个突出的吸收峰，阐明了 Ag-NPs 合成的构象。扫描电子显微镜（SEM）显示出独特的三角形、金字塔形和不规则形。X 射线衍射（XRD）证明了它们的结晶性质，Ag-NPs 的平均结晶粒度为 20.52 nm。傅立叶变换红外光谱（FT-IR）进一步证实了 Ag-NPs 表面存在酚（O-H 伸展）、过渡金属羰基 N-H、≡C-H、C ≡N、C ≡C、C-Cl、C-Br 和 O-H 键等官能团。Ag-NPs 对枯草芽孢杆菌的抗菌活性最为明显，在浓度为 1000 μg/mL 时，抑制区（ZOI）为 11 mm ± 0.57（抑制率为 45%）。同样，在 100 μg/mL 浓度下，Ag-NPs 的抗氧化潜力最高（73.2%），而在相同浓度下，标准抗坏血酸的抗氧化潜力为 76%。此外，在高浓度（200-300 ppm）下，柴胡介导的 Ag-NPs 对小麦种子生长和发芽的影响最大。总之，由 D. bupleuroides 介导的 Ag-NPs 的合成安全、经济、环保，可用作抗菌剂和抗氧化剂，并能提高全球农作物种子的生长和发芽率。研究亮点：纳米技术是对纳米粒子在生物和环境领域应用的研究。Ag-NPs 与其他纳米粒子一样，因其独特的性质而受到广泛关注。D. bupleuroides Ag-NPs: 45% 抗菌，73.2% 抗氧化，提高小麦发芽率。由 D. bupleuroides 介导的 Ag-NPs 既经济又环保。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.