Tuning almond lipase features by the buffer used during immobilization: The apparent biocatalysts stability depends on the immobilization and inactivation buffers and the substrate utilized

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Oumaima Cherni , Diego Carballares , El Hocine Siar , Pedro Abellanas-Perez , Diandra de Andrades , Maria de Lourdes Teixeira de Moraes Polizeli , Javier Rocha-Martin , Sellema Bahri , Roberto Fernandez-Lafuente
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引用次数: 0

Abstract

The lipase from Prunus dulcis almonds was inactivated under different conditions. At pH 5 and 9, enzyme stability remained similar under the different studied buffers. However, when the inactivation was performed at pH 7, there were some clear differences on enzyme stability depending on the buffer used. The enzyme was more stable in Gly than when Tris was employed for inactivation. Then, the enzyme was immobilized on methacrylate beads coated with octadecyl groups at pH 7 in the presence of Gly, Tris, phosphate and HEPES. Its activity was assayed versus triacetin and S-methyl mandelate. The biocatalyst prepared in phosphate was more active versus S-methyl mandelate, while the other ones were more active versus triacetin. The immobilized enzyme stability at pH 7 depends on the buffer used for enzyme immobilization. The buffer used in the inactivation and the substrate used determined the activity. For example, glycine was the buffer that promoted the lowest or the highest stabilities depending on the substrate used to quantify the activities.

通过固定过程中使用的缓冲液调整杏仁脂肪酶的特性:表面生物催化剂的稳定性取决于固定和失活缓冲液以及所使用的底物。
杏仁中的脂肪酶在不同条件下会失活。在 pH 值为 5 和 9 的条件下,不同研究的缓冲液对酶的稳定性保持相似。然而,在 pH 值为 7 的条件下进行灭活时,所使用的缓冲液不同,酶的稳定性也有明显差异。与使用 Tris 进行灭活相比,酶在 Gly 中的稳定性更高。然后,在 pH 值为 7 的 Gly、Tris、磷酸盐和 HEPES 溶液中,将酶固定在涂有十八烷基的甲基丙烯酸酯珠上。对三醋精和扁桃酸 S-甲酯的活性进行了检测。在磷酸盐中制备的生物催化剂对扁桃酸 S-甲基酯的活性更高,而其他生物催化剂对三醋精的活性更高。固定化酶在 pH 值为 7 时的稳定性取决于酶固定化所用的缓冲液。灭活所用的缓冲液和底物决定了酶的活性。例如,根据用于量化活性的底物,甘氨酸是促进最低或最高稳定性的缓冲液。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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