{"title":"Proposing new standards for testing solubility of pulp preservation materials","authors":"","doi":"10.1016/j.dental.2024.05.028","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>Quality control testing of dental materials requires a standard to enable the generation of reproducible and comparable data. Currently there are no standards for testing materials used for vital pulp therapy. The aim of this study was to develop a new standard to evaluate solubility of pulp preservation materials.</p></div><div><h3>Methods</h3><p>The solubility of three materials used for vital pulp therapy: Biodentine, TheraCal and Activa was evaluated using two international standards for dental materials ISO 4049:2019 (S1) and ISO 6876:2012 (S2). For both standards, a modified methodology was evaluated. This included changing the volume of the solution used (S1M, S2M), using Dulbecco’s modified eagle medium (DMEM) as an alternative to water (S1D, S2D) and periodic solution change for the ISO 4049 method (S1P, S1MP). Materials were characterised before and after completion of solubility test using scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis.</p></div><div><h3>Results</h3><p>The test materials exhibited different solubility values depending on the methodology used. Biodentine exhibited significantly lower solubility when lower volumes of solution were used when tested using both ISO methods (p ≤ 0.05). TheraCal and Activa showed negative solubility values after desiccation when tested using ISO 4049:2019. The Biodentine exhibited changes in its microstructure which was dependent on the method used to test solubility.</p></div><div><h3>Conclusions</h3><p>The solubility values obtained were dependent on the method used. It is thus important to use methods that replicate the clinical environment for meaningful evaluations.</p></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0109564124001295/pdfft?md5=bb5611d981b61d409b8a8c3ca013e82e&pid=1-s2.0-S0109564124001295-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dental Materials","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0109564124001295","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives
Quality control testing of dental materials requires a standard to enable the generation of reproducible and comparable data. Currently there are no standards for testing materials used for vital pulp therapy. The aim of this study was to develop a new standard to evaluate solubility of pulp preservation materials.
Methods
The solubility of three materials used for vital pulp therapy: Biodentine, TheraCal and Activa was evaluated using two international standards for dental materials ISO 4049:2019 (S1) and ISO 6876:2012 (S2). For both standards, a modified methodology was evaluated. This included changing the volume of the solution used (S1M, S2M), using Dulbecco’s modified eagle medium (DMEM) as an alternative to water (S1D, S2D) and periodic solution change for the ISO 4049 method (S1P, S1MP). Materials were characterised before and after completion of solubility test using scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis.
Results
The test materials exhibited different solubility values depending on the methodology used. Biodentine exhibited significantly lower solubility when lower volumes of solution were used when tested using both ISO methods (p ≤ 0.05). TheraCal and Activa showed negative solubility values after desiccation when tested using ISO 4049:2019. The Biodentine exhibited changes in its microstructure which was dependent on the method used to test solubility.
Conclusions
The solubility values obtained were dependent on the method used. It is thus important to use methods that replicate the clinical environment for meaningful evaluations.
目的:牙科材料的质量控制测试需要一个标准,以便生成可重复和可比较的数据。目前还没有用于检测牙髓治疗材料的标准。本研究的目的是制定一个新的标准来评估牙髓保存材料的溶解度:方法:对用于牙髓治疗的三种材料进行溶解度测试:方法:使用 ISO 4049:2019 (S1) 和 ISO 6876:2012 (S2) 两项牙科材料国际标准评估了 Biodentine、TheraCal 和 Activa 这三种用于牙髓治疗的材料的溶解度。针对这两项标准,采用了一种改进的方法进行评估。这包括改变所用溶液的体积(S1M、S2M),使用杜氏改良鹰培养基(DMEM)代替水(S1D、S2D),以及定期更换 ISO 4049 方法的溶液(S1P、S1MP)。利用扫描电子显微镜(SEM)和 X 射线衍射(XRD)分析法对溶解度测试前后的材料进行了表征:结果:根据所用方法的不同,测试材料表现出不同的溶解度值。在使用两种 ISO 方法进行测试时,如果使用的溶液量较少,则生物丹碱的溶解度明显较低(p ≤ 0.05)。使用 ISO 4049:2019 进行测试时,TheraCal 和 Activa 在干燥后的溶解度值为负值。Biodentine 的微观结构发生了变化,这与测试溶解度的方法有关:所获得的溶解度值取决于所使用的方法。因此,使用能复制临床环境的方法进行有意义的评估非常重要。
期刊介绍:
Dental Materials publishes original research, review articles, and short communications.
Academy of Dental Materials members click here to register for free access to Dental Materials online.
The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology.
Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.