Prothrombin and activated partial thromboplastin times, thromboelastography, hematocrit, and platelet count in a feline hemorrhage/over-resuscitation model using lactated Ringer's solution or 6% tetrastarch 130/0.4

IF 1.1 3区农林科学 Q3 VETERINARY SCIENCES

Journal of veterinary emergency and critical care Pub Date : 2024-06-14 DOI:10.1111/vec.13376

Gareth E. Zeiler BVSc(Hons), MMedVet(Anaesth), PhD, DECVECC, DECVAA, DACVAA, Brighton T. Dzikiti BVSc, MSc, PhD, Eva Rioja BVSc, DVM, PhD, DACVAA, Peter Kamerman BSc, PhD, Roxanne K. Buck BVSc, MMedVet(Anaesth), DECVAA, Friederike Pohlin BVSc, PhD, Andrea Fuller BSc, PhD

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引用次数: 0

Abstract

Objective

To describe and compare prothrombin time (PT), activated partial thromboplastin time (aPTT), thromboelastography (TEG), HCT, and platelet count measurements in a hemorrhage/over-resuscitation model.

Design

Randomized crossover study.

Setting

University teaching hospital.

Animals

Six cats.

Interventions

Anesthetized cats underwent 3 treatments at 2-month intervals. The treatments were as follows: NHR—no controlled hemorrhage and sham resuscitation; LRS—controlled hemorrhage and lactated Ringer's solution (LRS) for resuscitation; and Voluven—controlled hemorrhage and 6% tetrastarch 130/0.4 for resuscitation. The LRS and Voluven were administered at 60 and 20 mL/kg/h, respectively, for 120 minutes. Blood samples were drawn for PT, aPTT, TEG, HCT, and platelet count measurements at a healthy check (T − 7d), after controlled hemorrhage (T0), at 60 and 120 minutes of resuscitation (T60 and T120), and at 24 hours after completion of resuscitation (T24h). Data were analyzed using a general linear mixed model approach (significance was P < 0.05).

Measurements and Main Results

Total median blood loss (controlled hemorrhage and blood sampling from T0 to T120) at T120 was 11.4, 31.0, and 30.8 mL/kg for NHR, LRS, and Voluven, respectively. PT and aPTT during LRS and Voluven were prolonged at T60 and T120 compared to NHR (P < 0.001). On TEG, the reaction time, kinetic time, and alpha-angle were within reference intervals for cats at all time points in all treatments, while maximum amplitude was less than the reference interval (40 mm) at T0, T60, and T120 during Voluven and at T60 and T120 during LRS compared to NHR (both P < 0.001). The HCT and platelet count were significantly lower at T60 and T120 during LRS and Voluven compared to NHR (P < 0.001).

Conclusions

Hypocoagulopathy was observed during hemorrhage and liberal fluid resuscitation. Prolongation of PT and aPPT and decreased clot strength may have been caused by hemodilution and platelet loss.

Abstract Image

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使用乳酸林格氏液或 6% tetrastarch 130/0.4 对猫出血/过度复苏模型进行凝血酶原和活化部分凝血活酶时间、血栓弹力图、血细胞比容和血小板计数的研究。

目的描述并比较出血/过度复苏模型中凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）、血栓弹力图（TEG）、HCT 和血小板计数的测量结果：随机交叉研究：动物：六只猫六只猫：干预措施：麻醉猫接受 3 次治疗，每次间隔 2 个月。治疗方法如下NHR-无控制出血和假复苏；LRS-控制出血和乳酸林格氏液（LRS）复苏；Voluven-控制出血和 6% 四淀粉 130/0.4 复苏。LRS和Voluven的给药速度分别为60毫升/千克/小时和20毫升/千克/小时，持续120分钟。在健康检查（T - 7d）、控制出血后（T0）、复苏 60 分钟和 120 分钟（T60 和 T120）以及复苏结束后 24 小时（T24h）抽取血样进行 PT、aPTT、TEG、HCT 和血小板计数测量。数据采用一般线性混合模型方法进行分析（显著性为 P 测量和主要结果：T120时，NHR、LRS和Voluven的总失血中位数（T0至T120的控制性出血和采血）分别为11.4、31.0和30.8 mL/kg。与 NHR 相比，LRS 和 Voluven 在 T60 和 T120 时的 PT 和 aPTT 延长（P 结论）：在大出血和大量液体复苏期间可观察到低凝血功能。PT 和 aPPT 的延长以及凝血强度的降低可能是由血液稀释和血小板丢失引起的。

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来源期刊

Journal of veterinary emergency and critical care 农林科学-兽医学

CiteScore

2.50

自引率

15.40%

发文量

121

审稿时长

18-36 weeks

期刊介绍： The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.