Tubulysin Production by the Dead Cells of Archangium gephyra KYC5002.

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2024-06-01 Epub Date: 2024-06-13 DOI:10.1007/s12275-024-00130-3
Seohui Park, Chaehyeon Park, Yujin Ka, Kyungyun Cho
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Abstract

Archangium gephyra KYC5002 produces tubulysins during the death phase. In this study, we aimed to determine whether dead cells produce tubulysins. Cells were cultured for three days until the verge of the death phase, disrupted via ultrasonication, incubated for 2 h, and examined for tubulysin production. Non-disrupted cells produced 0.14 mg/L of tubulysin A and 0.11 mg/L of tubulysin B. Notably, tubulysin A production was increased by 4.4-fold to 0.62 mg/L and that of tubulysin B was increased by 6.7-fold to 0.74 mg/L in the disrupted cells. The same increase in tubulysin production was observed when the cells were killed by adding hydrogen peroxide. However, when the enzymes were inactivated via heat treatment of the cultures at 65 °C for 30 min, no significant increase in tubulysin production due to cell death was observed. Reverse transcription-quantitative polymerase chain reaction analysis of tubB mRNA revealed that the expression levels of tubulysin biosynthetic enzyme genes increased during the death phase compared to those during the vegetative growth phase. Our findings suggest that A. gephyra produces biosynthetic enzymes and subsequently uses them for tubulysin production in the cell death phase or during cell lysis by predators.

Abstract Image

弓形虫 KYC5002 死细胞产生的管胞素
Archangium gephyra KYC5002在死亡阶段会产生管胞素。在本研究中,我们旨在确定死亡细胞是否会产生管胞素。将细胞培养三天直至濒临死亡期,用超声波破坏细胞,培养 2 小时,然后检测细胞是否产生管胞素。未破坏的细胞产生 0.14 mg/L 的管胞素 A 和 0.11 mg/L 的管胞素 B。值得注意的是,在破坏的细胞中,管胞素 A 的产量增加了 4.4 倍,达到 0.62 mg/L,管胞素 B 的产量增加了 6.7 倍,达到 0.74 mg/L。当加入过氧化氢杀死细胞时,也观察到了同样的管胞素产量增加。然而,在 65 °C 下对培养物进行 30 分钟的热处理使酶失活时,没有观察到细胞死亡导致的管胞素产量的显著增加。对 tubB mRNA 的反转录定量聚合酶链反应分析表明,与无性生殖阶段相比,死亡阶段的管胞素生物合成酶基因表达水平有所提高。我们的研究结果表明,A. gephyra 能产生生物合成酶,然后在细胞死亡期或细胞被捕食者溶解时将其用于生产管胞素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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