Application of hydrophobic eutectic solvent in efficient biotransformation of total flavonoids of Herba Epimedii

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Qi Li , Shan Lu , Xianyao Wu , Lei Wang , Zhenzhong Wang , Linguo Zhao
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引用次数: 0

Abstract

Icaritin, a hydrolysate from total flavonoids of Epimedii (TFE), which has better anti-hepatoma activity than its glycosylated form. In this work, immobilized enzymes 4LP-Tpebgl3@Na-Y and DtRha@ES-107 were used to hydrolyze TFE to prepare icaritin. Five different hydrophobic deep eutectic solvents (HDES) were prepared and the most ideal HDES was successfully selected, which was composed of dodecyl alcohol and thymol with the molar ratio of 2:1. The relative enzyme activity of 4LP-Tpebgl3@Na-Y and DtRha@ES-107 was about 102.4 % and 112.5 %, respectively. In addition, the thermal and binding stability of 4LP-Tpebgl3@Na-Y and DtRha@ES-107 in HDES was not affected negatively. In the biphasic system composed of 50 % (v/v) HDES and Na2HPO4-citric acid buffer (50 mM, pH 5.5), 4LP-Tpebgl3@Na-Y (1.0 U/mL) and TFE (1 g/L) were reacted at 80 °C for 1 h, and then reacted with DtRha@ES-107 (20 U/mL) at 80 °C for 2 h. Finally, TFE was completely converted to 301.8 mg/L icaritin (0.82 mM). After 10 cycles, 4LP-Tpebgl3@Na-Y/DtRha@ES-107 still maintained 84.1 % original activity. In this study, we developed an efficient methodology for icaritin preparation through the integration of enzymatic catalysis and adsorption separation, presenting a viable approach for large-scale, cost-effective production of icaritin.

Abstract Image

疏水共晶溶剂在淫羊藿总黄酮高效生物转化中的应用
淫羊藿苷是从淫羊藿总黄酮(TFE)中提取的一种水解物,其抗肝癌活性优于其糖基化形式。在这项工作中,固定化酶 4LP-Tpebgl3@Na-Y 和 DtRha@ES-107 被用来水解 TFE 以制备水淫羊藿黄素。制备了五种不同的疏水性深共晶溶剂(HDES),并成功地选择了最理想的HDES,即由十二醇和百里酚组成,摩尔比为2:1。4LP-Tpebgl3@Na-Y和DtRha@ES-107的相对酶活分别约为102.4%和112.5%。此外,4LP-Tpebgl3@Na-Y 和 DtRha@ES-107 在 HDES 中的热稳定性和结合稳定性也没有受到负面影响。在由 50% (v/v) HDES 和 Na2HPO4-Citric acid 缓冲液(50mM,pH 5.5)组成的双相体系中,4LP-Tpebgl3@Na-Y(1.0U/mL)和 TFE(1g/L)在 80oC 下反应 1 小时,然后与 DtRha@ES-107 (20U/mL)在 80oC 下反应 2 小时。最后,TFE 被完全转化为 301.8mg/L 冰醋酸(0.82mM)。经过 10 个循环后,4LP-Tpebgl3@Na-Y/DtRha@ES-107 仍然保持了 84.1% 的原始活性。在这项研究中,我们通过酶催化与吸附分离的结合,开发出了一种高效的冰醋酸制备方法,为大规模、经济高效地生产冰醋酸提供了一种可行的途径。
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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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