Novel Serum Proteomes Expressed from Benzene Exposure Among Gasoline Station Attendants.

IF 3.4 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Biomarker Insights Pub Date : 2024-06-10 eCollection Date: 2024-01-01 DOI:10.1177/11772719241259604
Chan Pattama Polyong, Sittiruk Roytrakul, Jintana Sirivarasai, Tanongsak Yingratanasuk, Anamai Thetkathuek
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引用次数: 0

Abstract

Background: Research on the proteomes impact of benzene exposure in fuel station employees remains sparse, underscoring the need for detailed health impact assessments focusing on biomarker evaluation.

Objectives: This investigation aimed to analyze the differences in blood parameters and serum proteomes resulting from benzene exposure between gasoline station attendants (B-GSA) and a control group.

Design and methods: A cross-sectional analytical study was conducted with 96 participants, comprising 54 in the B-GSA group and 42 in the control group. The methodology employed included an interview questionnaire alongside urine and blood sample collections. The urine samples were analyzed for trans,trans-muconic acid (t,t-MA) levels, while the blood samples underwent complete blood count analysis and proteome profiling.

Results: Post-shift analysis indicated that the B-GSA group exhibited significantly higher levels of t,t-MA and monocytes compared to the control group (P < .05). Proteome quantification identified 1448 proteins differentially expressed between the B-GSA and control groups. Among these, 20 proteins correlated with the levels of t,t-MA in urine. Notably, 4 proteins demonstrated more than a 2-fold down-regulation in the B-GSA group: HBS1-like, non-structural maintenance of chromosomes element 1 homolog, proprotein convertase subtilisin/kexin type 4, and zinc finger protein 658. The KEGG pathway analysis revealed associations with apoptosis, cancer pathways, p53 signaling, and the TNF signaling pathway.

Conclusion: The changes in these 4 significant proteins may elucidate the molecular mechanisms underlying benzene toxicity and suggest their potential as biomarkers for benzene poisoning in future assessments.

加油站服务员因接触苯而表达的新血清蛋白质组
背景:有关苯暴露对加油站员工的蛋白质组影响的研究仍然很少,这突出表明需要以生物标志物评估为重点进行详细的健康影响评估:本调查旨在分析加油站员工(B-GSA)与对照组之间因接触苯而导致的血液参数和血清蛋白质组的差异:对 96 名参与者进行了横断面分析研究,其中 54 人属于 B-GSA 组,42 人属于对照组。采用的方法包括采访问卷以及尿样和血样采集。对尿样进行了反式、反式粘多酸(t,t-MA)水平分析,对血样进行了全血细胞计数分析和蛋白质组分析:转移后分析表明,与对照组相比,B-GSA 组的 t,t-MA和单核细胞水平明显更高(尿液中的 t,t-MA,P<0.05)。值得注意的是,有 4 种蛋白质在 B-GSA 组中的下调幅度超过 2 倍:HBS1-like、染色体非结构维持元件 1 同源物、4 型枯草蛋白/kexin 丙蛋白转换酶和 658 锌指蛋白。KEGG 通路分析显示,这些蛋白与细胞凋亡、癌症通路、p53 信号转导和 TNF 信号转导通路有关:结论:这 4 种重要蛋白质的变化可能会阐明苯中毒的分子机制,并表明它们在未来的评估中可能成为苯中毒的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomarker Insights
Biomarker Insights MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.00
自引率
0.00%
发文量
26
审稿时长
8 weeks
期刊介绍: An open access, peer reviewed electronic journal that covers all aspects of biomarker research and clinical applications.
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