Kigelia africana fruit fractions inhibit in vitro alpha-glucosidase activity: a potential natural alpha-glucosidase inhibitor.

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Tumelo Akapelwa Muyenga, Samuel K Dominion Bamitale, Dan Kibuule, Simbarashe Sithole, Stanley Mukanganyama, Carlen Rudolph, Luanne Venables, Anna C Hattingh, Maryna van de Venter, Christian Chinyere Ezeala
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Abstract

Background: Diabetes affects 75% of people in low-income countries, where conventional drugs like metformin are available, but newer drugs like alpha-glucosidase inhibitors are not accessible to most Southern African patients.

Aim: To evaluate the α-glucosidase and α-amylase inhibitory activities of fractionated aqueous extracts of Kigelia africana fruit (KAFE) and their phytochemical fingerprints using gas chromatography-mass spectrometry (GC-MS).

Materials and methods: We studied K. africana fruit fractions' inhibitory effects on alpha-glucosidase and alpha-amylase using bioassay-guided fractionation, and analyzed their phytochemical profiles with GC-MS.

Key findings: Both the aqueous extract and ethyl acetate fraction of the aqueous extract exhibited a low dose-dependent inhibition of alpha-amylase activity (p < 0.0001). At a concentration of 500 μg/mL, the aqueous extract caused an alpha-glucosidase inhibition of 64.10 ± 2.7%, with an estimated IC50 of 193.7 μg/mL, while the ethyl acetate fraction had an inhibition of 89.82 ± 0.8% and an estimated IC50 of 10.41 μg/mL. The subfraction G, which had the highest alpha-glucosidase inhibitory activity at 85.10 ± 0.7%, had significantly lower activity than the ethyl acetate fraction. The most bioactive fraction was found to contain 11"(2-cyclopenten-1-yl) undecanoic acid, ( +)- and cyclopentane undecanoic acid as well as the indole alkaloids Akuammilan-17-ol-10-methoxy, N-nitroso-2-methyl-oxazolidine and epoxide Oxirane2.2″ -(1.4-butanediyl) bis-.

Conclusion: The K. africana fruit fraction demonstrated significant alpha-glucosidase inhibitory activity, while its alpha-amylase inhibitory activity was limited. This study suggests a potential natural alpha-glucosidase inhibitor and phytocompounds that could serve as leads for developing antidiabetic agents.

Abstract Image

Kigelia africana 果实馏分抑制体外α-葡萄糖苷酶活性:一种潜在的天然α-葡萄糖苷酶抑制剂。
背景:在低收入国家,75% 的人患有糖尿病:目的:评估非洲鸢尾果(KAFE)水提取物的α-葡萄糖苷酶和α-淀粉酶抑制活性,并使用气相色谱-质谱法(GC-MS)分析其植物化学指纹:我们采用生物测定指导分馏法研究了非洲鸢尾果实馏分对α-葡萄糖苷酶和α-淀粉酶的抑制作用,并利用气相色谱-质谱法分析了它们的植物化学特征:主要发现:水提取物和水提取物的乙酸乙酯馏分对α-淀粉酶活性的抑制作用呈低剂量依赖性(p 结论:水提取物和乙酸乙酯馏分对α-淀粉酶活性的抑制作用呈低剂量依赖性(p 结论):K. africana果实馏分具有显著的α-葡萄糖苷酶抑制活性,而其α-淀粉酶抑制活性有限。这项研究提出了一种潜在的天然α-葡萄糖苷酶抑制剂和植物化合物,可作为开发抗糖尿病药物的线索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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