The intricacies of isomiRs: from classification to clinical relevance.

IF 13.6 2区 生物学 Q1 GENETICS & HEREDITY
Trends in Genetics Pub Date : 2024-09-01 Epub Date: 2024-06-10 DOI:10.1016/j.tig.2024.05.007
Viktoria Wagner, Eckart Meese, Andreas Keller
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引用次数: 0

Abstract

MicroRNAs (miRNAs) and isoforms of their archetype, called isomiRs, regulate gene expression via complementary base-pair binding to messenger RNAs (mRNAs). The partially evolutionarily conserved isomiR sequence variations are differentially expressed among tissues, populations, and genders, and between healthy and diseased states. Aiming towards the clinical use of isomiRs as diagnostic biomarkers and for therapeutic purposes, several challenges need to be addressed, including (i) clarification of isomiR definition, (ii) improved annotation in databases with new standardization (such as the mirGFF3 format), and (iii) improved methods of isomiR detection, functional verification, and in silico analysis. In this review we discuss the respective challenges, and highlight the opportunities for clinical use of isomiRs, especially in the light of increasing amounts of next-generation sequencing (NGS) data.

等位基因的复杂性:从分类到临床意义。
微小核糖核酸(miRNA)及其原型的异构体(称为 isomiRs)通过与信使核糖核酸(mRNA)的碱基对互补结合来调节基因表达。部分进化保守的 isomiR 序列变异在不同组织、人群、性别以及健康和疾病状态之间有不同的表达。为了将等位基因作为诊断生物标志物或用于临床治疗,需要解决几个难题,包括:(i) 明确等位基因的定义;(ii) 通过新的标准化(如 mirGFF3 格式)改进数据库中的注释;(iii) 改进等位基因的检测、功能验证和硅学分析方法。在这篇综述中,我们将讨论各自面临的挑战,并强调等位基因临床应用的机遇,尤其是在下一代测序(NGS)数据量不断增加的情况下。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Trends in Genetics
Trends in Genetics 生物-遗传学
CiteScore
20.90
自引率
0.90%
发文量
160
审稿时长
6-12 weeks
期刊介绍: Launched in 1985, Trends in Genetics swiftly established itself as a "must-read" for geneticists, offering concise, accessible articles covering a spectrum of topics from developmental biology to evolution. This reputation endures, making TiG a cherished resource in the genetic research community. While evolving with the field, the journal now embraces new areas like genomics, epigenetics, and computational genetics, alongside its continued coverage of traditional subjects such as transcriptional regulation, population genetics, and chromosome biology. Despite expanding its scope, the core objective of TiG remains steadfast: to furnish researchers and students with high-quality, innovative reviews, commentaries, and discussions, fostering an appreciation for advances in genetic research. Each issue of TiG presents lively and up-to-date Reviews and Opinions, alongside shorter articles like Science & Society and Spotlight pieces. Invited from leading researchers, Reviews objectively chronicle recent developments, Opinions provide a forum for debate and hypothesis, and shorter articles explore the intersection of genetics with science and policy, as well as emerging ideas in the field. All articles undergo rigorous peer-review.
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