Evaluation of bovine coronavirus in Korean native calves challenged through different inoculation routes.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Hyung-Chul Cho, Youngjun Kim, Yong-Il Cho, Jinho Park, Kyoung-Seong Choi
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引用次数: 0

Abstract

Bovine coronavirus (BCoV) is a pneumoenteric virus that can infect the digestive and respiratory tracts of cattle, resulting in economic losses. Despite its significance, information regarding BCoV pathogenesis is limited. Hence, we investigated clinical signs, patterns of viral shedding, changes in antibody abundance, and cytokine/chemokine production in calves inoculated with BCoV via intranasal and oral. Six clinically healthy Korean native calves (< 30 days old), initially negative for BCoV, were divided into intranasal and oral groups and monitored for 15 days post-infection (dpi). BCoV-infected calves exhibited clinical signs such as nasal discharge and diarrhea, starting at 3 dpi and recovering by 12 dpi, with nasal discharge being the most common symptoms. Viral RNA was detected in nasal and fecal samples from all infected calves. Nasal shedding occurred before fecal shedding regardless of the inoculation route; however, fecal shedding persisted longer. Although the number of partitions was very few, viral RNA was identified in the blood of two calves in the oral group at 7 dpi and 9 dpi using digital RT-PCR analysis. The effectiveness of maternal antibodies in preventing viral replication and shedding appeared limited. Our results showed interleukin (IL)-8 as the most common and highly induced chemokine. During BCoV infection, the levels of IL-8, monocyte chemoattractant protein-1, and macrophage inflammatory protein-1β were significantly affected, suggesting that these emerge as potential and reliable biomarkers for predicting BCoV infection. This study underscores the importance of BCoV as a major pathogen causing diarrhea and respiratory disease.

通过不同的接种途径对韩国本地犊牛的牛冠状病毒进行评估。
牛冠状病毒(BCoV)是一种肺肠道病毒,可感染牛的消化道和呼吸道,造成经济损失。尽管其意义重大,但有关 BCoV 发病机制的信息却很有限。因此,我们研究了经鼻和经口接种 BCoV 的犊牛的临床症状、病毒脱落模式、抗体丰度变化以及细胞因子/趋化因子的产生。六头临床健康的韩国本地小牛((
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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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