Testis-Specific Gene C7orf61 Is Involved in Mouse Sperm-Egg Fusion.

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

Urology Journal Pub Date : 2024-10-12 DOI:10.22037/uj.v21i.8073

Yong Wu, Zeng Zhang, Chihua He, Qiong Deng

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引用次数: 0

Abstract

Purpose: Chromosome 7 open reading frame 61 (C7orf 61) was a testis-specific gene, and may be involved in the process of spermatogenesis. This study aimed to investigate the expression of C7orf61 in the testis and determine its role in spermatogenesis.

Materials and methods: Reverse transcription-quantitative polymerase chain reaction, Western blot and immunofluorescence were performed to evaluate the expression characteristics of C7orf61 in mice and humans. In vitro fertilization assay was used to determine the role of the C7ORF61 protein in sperm-egg fusion.

Results: The results demonstrated that C7orf61 was a testis-specific gene; the C7ofr61 mRNA expression level sharply increased in the fourth postnatal week and gradually increased until the adult stage. The C7ORF61 protein was located throughout the subacrosomal area and close to the nucleus in both mouse and human sperm. The incubation with the C7ORF61 antibody significantly decreased the fertilization rate of mouse eggs.

Conclusion: The present findings suggested that the C7ORF61 protein might be involved in sperm-egg fusion, and could serve as a useful target for contraceptives. However, further research is still needed to know the detailed molecularmechanismofitsrole.

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睾丸特异基因 C7orf61 参与了小鼠精子与卵子的融合。

目的：染色体7开放阅读框61（C7orf 61）是睾丸特异性基因，可能参与精子发生过程。本研究旨在探讨C7orf61在睾丸中的表达，并确定其在精子发生过程中的作用。材料与方法采用逆转录-定量聚合酶链反应、Western印迹和免疫荧光技术评估C7orf61在小鼠和人类中的表达特征。体外受精试验用于确定 C7ORF61 蛋白在精卵融合中的作用：结果表明，C7orf61是一个睾丸特异性基因；C7ofr61 mRNA的表达水平在出生后第四周急剧上升，并在成年阶段逐渐增加。在小鼠和人类精子中，C7ORF61 蛋白位于整个顶体细胞下区域和细胞核附近。用 C7ORF61 抗体孵育小鼠精子可显著降低受精率：本研究结果表明，C7ORF61 蛋白可能参与了精卵融合，并可作为避孕药的有效靶点。结论：本研究结果表明，C7ORF61蛋白可能参与了精卵融合，可作为避孕药的有效靶标，但其作用的详细分子机制仍有待进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Urology Journal UROLOGY & NEPHROLOGY-

CiteScore

2.60

自引率

6.70%

发文量

审稿时长

6-12 weeks

期刊介绍： As the official journal of the Urology and Nephrology Research Center (UNRC) and the Iranian Urological Association (IUA), Urology Journal is a comprehensive digest of useful information on modern urology. Emphasis is on practical information that reflects the latest diagnostic and treatment techniques. Our objectives are to provide an exceptional source of current and clinically relevant research in the discipline of urology, to reflect the scientific work and progress of our colleagues, and to present the articles in a logical, timely, and concise format that meets the diverse needs of today’s urologist. Urology Journal publishes manuscripts on urology and kidney transplantation, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. Accordingly, original articles, case reports, and letters to editor are encouraged.