Azole Resistance in Veterinary Clinical Aspergillus fumigatus Isolates in the Netherlands.

IF 3.6 3区生物学 Q2 MYCOLOGY

Mycopathologia Pub Date : 2024-06-12 DOI:10.1007/s11046-024-00850-5

Marloes A M van Dijk, Jochem B Buil, Marlou Tehupeiory-Kooreman, Marian J Broekhuizen, Els M Broens, Jaap A Wagenaar, Paul E Verweij

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Abstract

Aspergillus fumigatus is a saprophytic fungal pathogen that causes opportunistic infections in animals and humans. Azole resistance has been reported globally in human A. fumigatus isolates, but the prevalence of resistance in isolates from animals is largely unknown. A retrospective resistance surveillance study was performed using a collection of clinical A. fumigatus isolates from various animal species collected between 2015 and 2020. Agar-based azole resistance screening of all isolates was followed by in vitro antifungal susceptibility testing and cyp51A gene sequencing of the azole-resistant isolates. Over the 5 year period 16 (11.3%) of 142 A. fumigatus culture-positive animals harbored an azole-resistant isolate. Resistant isolates were found in birds (15%; 2/13), cats (21%; 6/28), dogs (8%; 6/75) and free-ranging harbor porpoise (33%; 2/6). Azole-resistance was cyp51A mediated in all isolates: 81.3% (T-67G/)TR₃₄/L98H, 12.5% TR₄₆/Y121F/T289A. In one azole-resistant A. fumigatus isolate a combination of C(-70)T/F46Y/C(intron7)T/C(intron66)T/M172V/E427K single-nucleotide polymorphisms in the cyp51A gene was found. Of the animals with an azole-resistant isolate and known azole exposure status 71.4% (10/14) were azole naive. Azole resistance in A. fumigatus isolates from animals in the Netherlands is present and predominantly cyp51A TR-mediated, supporting an environmental route of resistance selection. Our data supports the need to include veterinary isolates in resistance surveillance programs. Veterinarians should consider azole resistance as a reason for therapy failure when treating aspergillosis and consider resistance testing of relevant isolates.

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荷兰兽医临床曲霉菌分离物中的唑类抗药性。

烟曲霉（Aspergillus fumigatus）是一种吸附性真菌病原体，可引起动物和人类的机会性感染。据报道，全球范围内的人类烟曲霉分离物都对唑类药物产生了抗药性，但动物分离物的抗药性流行情况却不为人知。我们利用 2015 年至 2020 年间从不同动物物种中收集到的临床烟曲霉分离株进行了一项回顾性耐药性监测研究。在对所有分离株进行琼脂基唑类耐药性筛选后，又对耐唑类分离株进行了体外抗真菌药敏试验和 cyp51A 基因测序。在 5 年的时间里，142 个烟曲霉培养阳性动物中有 16 个（11.3%）携带耐唑分离物。在鸟类（15%；2/13）、猫类（21%；6/28）、狗类（8%；6/75）和自由放养的港湾鼠海豚（33%；2/6）中都发现了耐药性分离株。所有分离物对唑类的抗药性都是由 cyp51A 介导的：81.3% (t-67g/)tr34/l98h, 12.5% tr46/y121f/t289a。在一个抗唑烟曲霉分离株中，发现了 cyp51A 基因中的 C(-70)T/F46Y/C(intron7)T/C(intron66)T/M172V/E427K 单核苷酸多态性组合。在分离出抗偶氮唑且已知偶氮唑暴露状态的动物中，71.4%（10/14）对偶氮唑不敏感。荷兰动物中分离出的烟曲霉菌具有唑耐药性，且主要由 cyp51A TR 介导，这支持了耐药性选择的环境途径。我们的数据支持将兽医分离物纳入耐药性监测计划的必要性。兽医在治疗曲霉菌病时应将唑类耐药性视为治疗失败的原因之一，并考虑对相关分离物进行耐药性检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycopathologia 生物-真菌学

CiteScore

6.80

自引率

3.60%

发文量

审稿时长

3 months

期刊介绍： Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.