Amino acid residues responsible for the different pH dependency of cell-specific ferredoxins in the electron transfer reaction with ferredoxin-NADP+ reductase from maize leaves.

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yoko Kimata-Ariga, Hikaru Tanaka, Shunsuke Kuwano
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引用次数: 0

Abstract

In the chloroplast stroma, dynamic pH changes occur from acidic to alkaline in response to fluctuating light conditions. We investigated the pH dependency of the electron transfer reaction of ferredoxin-NADP+ reductase (FNR) with ferredoxin (Fd) isoproteins, Fd1 and Fd2, which are localized in mesophyll cells and bundle sheath cells, respectively, in the leaves of C4 plant maize. The pH-dependent profile of the electron transfer activity with FNR was quite different between Fd1 and Fd2, which was mainly explained by the opposite pH dependency of the Km value of these Fds for FNR. Replacement of the amino acid residue at position of 65 (D65N) and 78 (H78A) between the two Fds conferred different effect on their pH dependency of the Km value. Double mutations of the two residues between Fd1 and Fd2 (Fd1D65N/H78A and Fd2N65D/A78H) led to the mutual exchange of the pH dependency of the electron transfer activity. This exchange was mainly explained by the changes in the pH-dependent profile of the Km values. Therefore, the differences in Asp/Asn at position 65 and His/Ala at position 78 between Fd1 and Fd2 were shown to be the major determinants for their different pH dependency in the electron transfer reaction with FNR.

在与玉米叶片中的铁氧还蛋白-NADP+还原酶进行电子转移反应时,细胞特异性铁氧还蛋白对 pH 值的依赖性不同的氨基酸残基。
在叶绿体基质中,pH值会随着光照条件的变化而发生从酸性到碱性的动态变化。我们研究了铁氧还蛋白-NADP+还原酶(FNR)与铁氧还蛋白(Fd)异构体Fd1和Fd2的电子传递反应的pH依赖性。Fd1 和 Fd2 与 FNR 的电子传递活性随 pH 值变化的曲线截然不同,这主要是因为这两种 Fds 对 FNR 的 Km 值与 pH 值的依赖性相反。两个 Fds 之间 65(D65N)和 78(H78A)位氨基酸残基的置换对其 Km 值的 pH 依赖性产生了不同的影响。Fd1 和 Fd2 之间两个残基的双突变(Fd1D65N/H78A 和 Fd2N65D/A78H)导致电子转移活性的 pH 依赖性相互交换。这种交换的主要原因是 Km 值的 pH 依赖性曲线发生了变化。因此,Fd1 和 Fd2 在与 FNR 进行电子转移反应时,其 65 位的 Asp/Asn 和 78 位的 His/Ala 的差异被证明是导致其 pH 依赖性不同的主要决定因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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