Enhancing drought tolerance in broccoli (Brassica oleracea L.) through melatonin application: Physiological and biochemical insights into growth, photosynthesis, and antioxidant defense mechanisms
Hasan Sardar , Muhammad Shafiq , Safina Naz , Sajid Ali , Riaz Ahmad , Shaghef Ejaz
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引用次数: 0
Abstract
Broccoli is a well-known and high-value vegetable crop for its abundance of antioxidants and minerals. Despite its popularity, its production faces significant challenges from various abiotic stressors, particularly drought stress. Melatonin a multifunctional molecule within plants modulates environmental stresses including drought stress. Therefore, the current study aimed to evaluate the effect of melatonin application (0 and 100 μM) on broccoli under 100% field capacity (FC) (well-watered) and drought stress (50% field capacity). Foliar application of melatonin helped overcome drought-induced losses and enhanced plant height (23.72%), root length (43.47%), total chlorophyll contents (46%), carotenoids (60%) and yield (34.23%), glycine betaine (GB) (77.7%), whereas reducing the oxidative activities of hydrogen peroxide (H2O2) (20%) and leaf water potential (LWP) (33.35%) in broccoli plants. Antioxidant activities, i.e., superoxide dismutase (SOD), peroxide (POD), catalase (CAT), and ascorbate (APX) were further enhanced under melatonin treatment with 28.53%, 26.10%, 35.26%, and 33.33% respectively. Melatonin application significantly increased gas exchange characteristics by increasing stomatal conductance. Pearson correlation matrix and biplot were developed for the determination of trait association and treatment performance. Exogenous melatonin application decreased the oxidative damage brought on by the drought and increased the yield of broccoli.
期刊介绍:
Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.