Carlos E B Lopes, Fabiana G Xavier, Rafael R Nicolino, Luana F M Cordeiro, Leandro C Rezende, Marcelo C Lopes, Dayse H L Silva, Antônio A Fonseca Júnior, Luciana R Ferreira, Marcelo F Camargos, Paulo M Soares Filho, Ivy C C Souza, Roselene Ecco
{"title":"Pathological findings and differential diagnoses of lymph node diseases in slaughtered cattle in Brazil: A study of 2000 samples.","authors":"Carlos E B Lopes, Fabiana G Xavier, Rafael R Nicolino, Luana F M Cordeiro, Leandro C Rezende, Marcelo C Lopes, Dayse H L Silva, Antônio A Fonseca Júnior, Luciana R Ferreira, Marcelo F Camargos, Paulo M Soares Filho, Ivy C C Souza, Roselene Ecco","doi":"10.1177/03009858241257908","DOIUrl":null,"url":null,"abstract":"<p><p>Slaughterhouse inspections play a crucial role in the sanitary control of zoonoses and foodborne diseases. This study aimed to identify and analyze the frequencies of lymph node diseases in cattle slaughtered for human consumption, using the samples sent to the anatomic pathology service of the Federal Laboratory for Agricultural Defense (<i>Laboratório Federal de Defesa Agropecuária</i>), Minas Gerais, Brazil, from January 2015 to September 2022. In total, 2000 lymph node samples were analyzed, and additional information was individually retrieved. Lesions were most frequently identified in thoracic lymph nodes. Bacterial isolation and quantitative polymerase chain reaction (qPCR) were performed using samples suspected of tuberculosis. Tuberculosis cases accounted for 89.3% of the samples. Histopathology was more sensitive than other ancillary tests for diagnosing tuberculosis. Paraffin-embedded tissues from lymphoma cases were subjected to immunophenotyping using anti-CD3 and anti-CD79a immunohistochemistry. Frozen and/or paraffin-embedded tissues from lymphoma cases were used to identify the enzootic bovine leukosis (EBL) retrovirus through qPCR. Other diagnoses included primary (T- and B-cell lymphoma) and metastatic neoplasms (squamous cell carcinoma, pulmonary adenocarcinoma, undifferentiated carcinoma, undifferentiated adenocarcinoma, undifferentiated sarcoma, undifferentiated round cell tumor, mesothelioma, hepatic carcinoid, meningioma, and seminoma), actinogranulomas (pyogranulomatous lymphadenitis [actinobacillosis and actinomycosis]), idiopathic lymphadenitis (neutrophilic and/or histiocytic, granulomatous, and suppurative), and miscellaneous nonspecific lymphadenopathies (depletion/lymphoid atrophy, lymphangiectasia, erythrocyte drainage, parasitic eosinophilic lymphadenitis, follicular hyperplasia, and toxic granulomatous lymphadenitis). The combination of histopathology with complementary techniques is important for successful diagnosis, especially in complex cases of high epidemiological, economic, and zoosanitary importance, such as tuberculosis and EBL.</p>","PeriodicalId":23513,"journal":{"name":"Veterinary Pathology","volume":" ","pages":"952-964"},"PeriodicalIF":2.3000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1177/03009858241257908","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/11 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PATHOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Slaughterhouse inspections play a crucial role in the sanitary control of zoonoses and foodborne diseases. This study aimed to identify and analyze the frequencies of lymph node diseases in cattle slaughtered for human consumption, using the samples sent to the anatomic pathology service of the Federal Laboratory for Agricultural Defense (Laboratório Federal de Defesa Agropecuária), Minas Gerais, Brazil, from January 2015 to September 2022. In total, 2000 lymph node samples were analyzed, and additional information was individually retrieved. Lesions were most frequently identified in thoracic lymph nodes. Bacterial isolation and quantitative polymerase chain reaction (qPCR) were performed using samples suspected of tuberculosis. Tuberculosis cases accounted for 89.3% of the samples. Histopathology was more sensitive than other ancillary tests for diagnosing tuberculosis. Paraffin-embedded tissues from lymphoma cases were subjected to immunophenotyping using anti-CD3 and anti-CD79a immunohistochemistry. Frozen and/or paraffin-embedded tissues from lymphoma cases were used to identify the enzootic bovine leukosis (EBL) retrovirus through qPCR. Other diagnoses included primary (T- and B-cell lymphoma) and metastatic neoplasms (squamous cell carcinoma, pulmonary adenocarcinoma, undifferentiated carcinoma, undifferentiated adenocarcinoma, undifferentiated sarcoma, undifferentiated round cell tumor, mesothelioma, hepatic carcinoid, meningioma, and seminoma), actinogranulomas (pyogranulomatous lymphadenitis [actinobacillosis and actinomycosis]), idiopathic lymphadenitis (neutrophilic and/or histiocytic, granulomatous, and suppurative), and miscellaneous nonspecific lymphadenopathies (depletion/lymphoid atrophy, lymphangiectasia, erythrocyte drainage, parasitic eosinophilic lymphadenitis, follicular hyperplasia, and toxic granulomatous lymphadenitis). The combination of histopathology with complementary techniques is important for successful diagnosis, especially in complex cases of high epidemiological, economic, and zoosanitary importance, such as tuberculosis and EBL.
屠宰场检查在人畜共患病和食源性疾病的卫生控制方面发挥着至关重要的作用。本研究旨在利用2015年1月至2022年9月期间送往巴西米纳斯吉拉斯州联邦农业防卫实验室(Laboratório Federal de Defesa Agropecuária)解剖病理学部门的样本,确定和分析供人类食用的屠宰牛的淋巴结疾病频率。共分析了2000份淋巴结样本,并逐一检索了其他信息。胸腔淋巴结最常出现病变。对疑似结核病的样本进行了细菌分离和定量聚合酶链反应(qPCR)。结核病例占样本的 89.3%。在诊断结核病方面,组织病理学比其他辅助检查更敏感。淋巴瘤病例的石蜡包埋组织采用抗-CD3和抗-CD79a免疫组化技术进行免疫分型。淋巴瘤病例的冷冻和/或石蜡包埋组织通过 qPCR 鉴定牛白血病(EBL)逆转录病毒。其他诊断包括原发性(T 细胞和 B 细胞淋巴瘤)和转移性肿瘤(鳞状细胞癌、肺腺癌、未分化癌、未分化腺癌、未分化肉瘤、未分化圆形细胞瘤、间皮瘤、肝类癌、脑膜瘤和精原细胞瘤)、放线菌瘤(化脓性淋巴结炎[放线菌病和放线菌病])、特发性淋巴结炎(嗜中性和/或组织细胞性、肉芽肿性和化脓性)、以及其他非特异性淋巴结病(淋巴腺耗竭/淋巴管萎缩、淋巴管扩张、红细胞引流、寄生虫性嗜酸性淋巴结炎、滤泡增生和中毒性肉芽肿淋巴结炎)。组织病理学与辅助技术的结合对成功诊断非常重要,尤其是对结核病和 EBL 等在流行病学、经济学和动物卫生学方面具有重要意义的复杂病例。
期刊介绍:
Veterinary Pathology (VET) is the premier international publication of basic and applied research involving domestic, laboratory, wildlife, marine and zoo animals, and poultry. Bridging the divide between natural and experimental diseases, the journal details the diagnostic investigations of diseases of animals; reports experimental studies on mechanisms of specific processes; provides unique insights into animal models of human disease; and presents studies on environmental and pharmaceutical hazards.