Effects of honokiol combined with resveratrol on bacteria responsible for oral malodor and their biofilm.

IF 3.7 2区医学 Q2 MICROBIOLOGY

Journal of Oral Microbiology Pub Date : 2024-06-09 eCollection Date: 2024-01-01 DOI:10.1080/20002297.2024.2361402

Shiqian Zheng, Rongrong Deng, Gengjiu Huang, Zhiwen Ou, Zhibin Shen

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引用次数: 0

Abstract

Background: This study aimed to investigate the effect of honokiol combined with resveratrol on bacteria responsible for oral malodor and their biofilm.

Method: This study investigated drug's MIC, FICI and dynamic bactericidal susceptibility activities against Pg and Fn. The effects of drugs on biofilm metabolic activity, biofilm total amount, and biofilm microstructure were determined by CCK-8 experiment, semi-quantitative adhesion experiment and SEM, respectively. The effects of drugs on biofilm genes, extracellular polysaccharides, proteins and DNA content were determined by qRT-PCR, phenol-sulfuric acid method, BCA method and Nano Drop one C, respectively.

Results: The combination had synergistic antibacterial effect on Pg and Fn. 1/2×MIC and 1×MIC combination inhibit the whole process of Pg and Fn growth. The results showed that the combination effectively reduce biofilm metabolic activity and total amount, and destroy biofilm microstructure. The results showed that the combination downregulate the gene expression both Pg and Fn, reduce extracellular polysaccharides and DNA of Pg, and reduce extracellular proteins and DNA of Fn.

Conclusion: This study showed that the combination had a synergistic antibacterial effect on Pg and Fn, reduced the biofilm extracellular matrix, inhibited biofilm formation, and downregulated the expression of genes related to biofilm formation.

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红景天和白藜芦醇对导致口腔异味的细菌及其生物膜的影响。

背景：本研究旨在探讨霍诺克醇联合白藜芦醇对口腔恶臭细菌及其生物膜的影响：本研究旨在探讨霍诺克醇与白藜芦醇复方制剂对导致口腔异味的细菌及其生物膜的影响：本研究调查了药物对 Pg 和 Fn 的 MIC、FICI 和动态杀菌敏感性。通过 CCK-8 实验、半定量粘附实验和扫描电镜分别测定了药物对生物膜代谢活性、生物膜总量和生物膜微结构的影响。通过 qRT-PCR、酚硫酸法、BCA 法和 Nano Drop one C 分别测定了药物对生物膜基因、胞外多糖、蛋白质和 DNA 含量的影响：结果：联合用药对 Pg 和 Fn 有协同抗菌作用。1/2×MIC 和 1×MIC 组合抑制了 Pg 和 Fn 的整个生长过程。结果表明，组合物能有效降低生物膜的代谢活性和总量，破坏生物膜的微结构。结果表明，该组合能下调 Pg 和 Fn 的基因表达，减少 Pg 的胞外多糖和 DNA，减少 Fn 的胞外蛋白和 DNA：本研究表明，联合用药对 Pg 和 Fn 有协同抗菌作用，能减少生物膜细胞外基质，抑制生物膜的形成，并能下调与生物膜形成相关的基因表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Microbiology MICROBIOLOGY-

CiteScore

8.00

自引率

4.40%

发文量

审稿时长

12 weeks

期刊介绍： As the first Open Access journal in its field, the Journal of Oral Microbiology aims to be an influential source of knowledge on the aetiological agents behind oral infectious diseases. The journal is an international forum for original research on all aspects of ''oral health''. Articles which seek to understand ''oral health'' through exploration of the pathogenesis, virulence, host-parasite interactions, and immunology of oral infections are of particular interest. However, the journal also welcomes work that addresses the global agenda of oral infectious diseases and articles that present new strategies for treatment and prevention or improvements to existing strategies. Topics: ''oral health'', microbiome, genomics, host-pathogen interactions, oral infections, aetiologic agents, pathogenesis, molecular microbiology systemic diseases, ecology/environmental microbiology, treatment, diagnostics, epidemiology, basic oral microbiology, and taxonomy/systematics. Article types: original articles, notes, review articles, mini-reviews and commentaries