Metabolomics efficiently discriminates monozygotic twins in peripheral blood.

IF 2.2 3区 医学 Q1 MEDICINE, LEGAL
International Journal of Legal Medicine Pub Date : 2024-11-01 Epub Date: 2024-06-11 DOI:10.1007/s00414-024-03269-1
Kuo Zeng, Jiang Du, Yun-Zhou Chen, Dan-Yang Wang, Mao-Ling Sun, Yu-Zhang Li, Dong-Yi Wang, Shu-Han Liu, Xiu-Mei Zhu, Peng Lv, Zhe Du, Kun Liu, Jun Yao
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引用次数: 0

Abstract

Monozygotic (MZ) twins cannot be distinguished using conventional forensic STR typing because they present identical STR genotypings. However, MZ twins do not always live in the same environment and often have different dietary and other lifestyle habits. Metabolic profiles are deyermined by individual characteristics and are also influenced by the environment in which they live. Therefore, they are potential markers capable of identifying MZ twins. Moreover, the production of proteins varies from organism to organism and is influenced by both the physiological state of the body and the external environment. Hence, we used metabolomics and proteomics to identify metabolites and proteins in peripheral blood to discriminate MZ twins. We identified 1749 known metabolites and 622 proteins in proteomic analysis. The metabolic profiles of four pairs of MZ twins revealed minor differences in intra-MZ twins and major differences in inter-MZ twins. Each pair of MZ twins exhibited distinct characteristics, and four metabolites-methyl picolinate, acesulfame, paraxanthine, and phenylbenzimidazole sulfonic acid-were observed in all four MZ twin pairs. These four differential exogenous metabolites conincidently show that the different external environments and life styles can be well distinguished by metabolites, considering that twins do not all have the same eating habits and living environments. Moreover, MZ twins showed different protein profiles in serum but not in whole blood. Thus, our results indicate that differential metabolites provide potential biomarkers for the personal identification of MZ twins in forensic medicine.

Abstract Image

代谢组学可有效区分外周血中的单卵双胞胎。
单卵双生(MZ)双胞胎无法通过传统的法医 STR 分型进行区分,因为他们的 STR 基因分型完全相同。然而,MZ 双胞胎并不总是生活在相同的环境中,而且往往有不同的饮食和其他生活习惯。代谢特征由个体特征决定,同时也受到生活环境的影响。因此,它们是能够识别 MZ 双胞胎的潜在标记物。此外,蛋白质的产生因生物体而异,并受到机体生理状态和外部环境的影响。因此,我们利用代谢组学和蛋白质组学来鉴定外周血中的代谢物和蛋白质,以鉴别 MZ 双胞胎。在蛋白质组学分析中,我们确定了 1749 种已知代谢物和 622 种蛋白质。四对 MZ 双胞胎的代谢图谱显示,MZ 内双胞胎的差异较小,而 MZ 间双胞胎的差异较大。每对 MZ 双胞胎都表现出不同的特征,在所有四对 MZ 双胞胎中都观察到了四种代谢物--吡啶甲酸甲酯、安赛蜜、对羟基黄嘌呤和苯并咪唑磺酸。这四种不同的外源性代谢物不约而同地表明,考虑到双胞胎的饮食习惯和生活环境不尽相同,不同的外部环境和生活方式可以通过代谢物很好地区分开来。此外,MZ 双胞胎在血清中显示出不同的蛋白质谱,但在全血中没有。因此,我们的研究结果表明,不同的代谢物为法医学中MZ双胞胎的个人识别提供了潜在的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.80
自引率
9.50%
发文量
165
审稿时长
1 months
期刊介绍: The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.
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