Hongyan Xu, Wenzhuo Ban, Jiaming Tian, Jianfei Xu, Zhimin Tan, Sendong Li, Kaili Chen, Mi Ou, Kaibin Li
{"title":"The New Roles of traf6 Gene Involved in the Development of Zebrafish Liver and Gonads","authors":"Hongyan Xu, Wenzhuo Ban, Jiaming Tian, Jianfei Xu, Zhimin Tan, Sendong Li, Kaili Chen, Mi Ou, Kaibin Li","doi":"10.1007/s10126-024-10329-5","DOIUrl":null,"url":null,"abstract":"<div><p>Traf6, an adaptor protein, exhibits non-conventional E3 ubiquitin ligase activity and was well studied as an important factor in immune systems and cancerogenesis. In mice, the traf6-null caused a perinatal death, so that the underlying pathophysiology of <i>traf6</i>-defeciency is still largely unclear in animals. Here, in the present study, a <i>traf6</i> knockout zebrafish line (<i>traf6</i><sup><i>−/−</i></sup>) was generated and could survive until adulthood, providing a unique opportunity to demonstrate the functions of <i>traf6</i> gene in animals’ organogenesis beyond the mouse model. The body of <i>traf6</i><sup><i>−/−</i></sup> fish was found to be significantly shorter than that of the wildtype (WT). Likewise, a comparative transcriptome analysis showed that 866 transcripts were significantly altered in the <i>traf6</i><sup><i>−/−</i></sup> liver, mainly involved in the immune system, metabolic pathways, and progesterone-mediated oocyte maturation. Especially, the mRNA expression of the pancreas duodenum homeobox protein 1 (<i>pdx1</i>), glucose-6-phosphatase (<i>g6pcb</i>), and the vitellogenesis genes (<i>vtgs</i>) were significantly decreased in the <i>traf6</i><sup><i>−/−</i></sup> liver. Subsequently, the glucose was found to be accumulated in the <i>traf6</i><sup><i>−/−</i></sup> liver tissues, and the meiotic germ cell was barely detected in <i>traf6</i><sup><i>−/−</i></sup> testis or ovary. The findings of this study firstly implied the pivotal functions of <i>traf6</i> gene in the liver and gonads’ development in fish species.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"917 - 930"},"PeriodicalIF":2.6000,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10126-024-10329-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Traf6, an adaptor protein, exhibits non-conventional E3 ubiquitin ligase activity and was well studied as an important factor in immune systems and cancerogenesis. In mice, the traf6-null caused a perinatal death, so that the underlying pathophysiology of traf6-defeciency is still largely unclear in animals. Here, in the present study, a traf6 knockout zebrafish line (traf6−/−) was generated and could survive until adulthood, providing a unique opportunity to demonstrate the functions of traf6 gene in animals’ organogenesis beyond the mouse model. The body of traf6−/− fish was found to be significantly shorter than that of the wildtype (WT). Likewise, a comparative transcriptome analysis showed that 866 transcripts were significantly altered in the traf6−/− liver, mainly involved in the immune system, metabolic pathways, and progesterone-mediated oocyte maturation. Especially, the mRNA expression of the pancreas duodenum homeobox protein 1 (pdx1), glucose-6-phosphatase (g6pcb), and the vitellogenesis genes (vtgs) were significantly decreased in the traf6−/− liver. Subsequently, the glucose was found to be accumulated in the traf6−/− liver tissues, and the meiotic germ cell was barely detected in traf6−/− testis or ovary. The findings of this study firstly implied the pivotal functions of traf6 gene in the liver and gonads’ development in fish species.
期刊介绍:
Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.