Analysis of MiR-20b, MIR-197 markers for differentiation between forensic body fluids encountered in sexual assault cases.

IF 1.5 4区 医学 Q2 MEDICINE, LEGAL
Forensic Science, Medicine and Pathology Pub Date : 2025-03-01 Epub Date: 2024-06-10 DOI:10.1007/s12024-024-00831-6
Shimaa Ahmed Alsaeed, Noha Maher Elrewieny, Rabab Abdulmoez Amin Eltokhy, Mohamed Shokr Mohamed, Wagdy K B Khalil, Aziza B Shalby, Hoda F Booles, Heba Mohamed Aboubakr
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Abstract

Identifying body fluids can be a critical clue that aids in reconstructing the crime scene. Semen and vaginal fluid identification is crucial, especially in cases of sexual assault. The majority of forensic studies focused on identifying normal body fluids and neglected the expression variation of semen in pathology. To differentiate between vaginal fluids, fertile and infertile semen samples (oligospermia and azoospermia) using miR 20b and miR197. A total of 48 body fluid samples, divided as 16 vaginal fluids, 16 fertile semen, and 16 infertile semen samples (8 with oligospermia and 8 with azoospermia), were collected, and the expression levels of miR-20b and miR-197 were detected by the SYBR Green real-time quantitative PCR technique. Our results showed significant different expression of these miRNAs in normal semen compared to vaginal and infertile semen. Moreover, we designed a model based on Fisher's discriminant function to forecast the group affiliations of unidentified samples. With three novel equations, we were able to accurately distinguish between semen and vaginal fluid, fertile and infertile semen, and oligospermia and azoospermia semen samples with validation accuracy of 81.3%, 100%, and 100%, respectively. MiR-20b and miR-197 expression levels are efficient and appropriate markers to distinguish semen from vaginal fluid and to differentiate between fertile and infertile semen samples. However, the present study is a preliminary study based on clinical samples, and the potential role of these markers in differentiating real crime scene samples is still unknown, so we recommend further research to investigate these markers expression while using forensic samples.

Abstract Image

分析 MiR-20b、MIR-197 标记,以区分性侵犯案件中遇到的法医体液。
体液鉴定是帮助重建犯罪现场的重要线索。精液和阴道分泌物的鉴定至关重要,尤其是在性侵犯案件中。大多数法医研究侧重于识别正常体液,而忽视了精液在病理学中的表达变化。为了区分阴道液、可育和不可育精液样本(少精子症和无精子症),使用了 miR 20b 和 miR197。研究人员共采集了48份体液样本,分为16份阴道液样本、16份可育精液样本和16份不育精液样本(8份少精症样本和8份无精症样本),并采用SYBR Green实时定量PCR技术检测了miR-20b和miR-197的表达水平。结果表明,与阴道精液和不育精液相比,正常精液中这些 miRNA 的表达量有明显差异。此外,我们还设计了一个基于费雪判别函数的模型来预测未识别样本的组别归属。通过三个新方程,我们能够准确地区分精液和阴道液、可育精液和不育精液、少精子症和无精子症精液样本,验证准确率分别为 81.3%、100% 和 100%。MiR-20b和miR-197的表达水平是区分精液和阴道液以及区分可育和不育精液样本的有效而适当的标记物。然而,本研究只是基于临床样本的初步研究,这些标记物在区分真实犯罪现场样本中的潜在作用尚不清楚,因此我们建议在使用法医样本时进一步研究这些标记物的表达。
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来源期刊
Forensic Science, Medicine and Pathology
Forensic Science, Medicine and Pathology MEDICINE, LEGAL-PATHOLOGY
CiteScore
3.90
自引率
5.60%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Forensic Science, Medicine and Pathology encompasses all aspects of modern day forensics, equally applying to children or adults, either living or the deceased. This includes forensic science, medicine, nursing, and pathology, as well as toxicology, human identification, mass disasters/mass war graves, profiling, imaging, policing, wound assessment, sexual assault, anthropology, archeology, forensic search, entomology, botany, biology, veterinary pathology, and DNA. Forensic Science, Medicine, and Pathology presents a balance of forensic research and reviews from around the world to reflect modern advances through peer-reviewed papers, short communications, meeting proceedings and case reports.
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