Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells.

IF 3.2 3区 生物学 Q2 BIOPHYSICS
Biophysical journal Pub Date : 2025-06-03 Epub Date: 2024-06-08 DOI:10.1016/j.bpj.2024.06.007
Tomasz J Nawara, Jie Yuan, Leslie D Seeley, Elizabeth Sztul, Alexa L Mattheyses
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引用次数: 0

Abstract

Endothelial cells (ECs) experience a variety of highly dynamic mechanical stresses. Among others, cyclic stretch and increased plasma membrane tension inhibit clathrin-mediated endocytosis (CME) in non-ECs. It remains elusive how ECs maintain CME in these biophysically unfavorable conditions. Previously, we have used simultaneous two-wavelength axial ratiometry (STAR) microscopy to show that endocytic dynamics are similar between statically cultured human umbilical vein endothelial cells (HUVECs) and fibroblast-like Cos-7 cells. Here, we asked whether biophysical stresses generated by blood flow influence CME. We used our data processing platform-DrSTAR-to examine if clathrin dynamics are altered in HUVECs after experiencing fluidic shear stress (FSS). We found that HUVECs cultivated under a physiological level of FSS had increased clathrin dynamics compared with static controls. FSS increased both clathrin-coated vesicle formation and nonproductive flat clathrin lattices by 2.3-fold and 1.9-fold, respectively. The curvature-positive events had significantly delayed curvature initiation relative to clathrin recruitment in flow-stimulated cells, highlighting a shift toward flat-to-curved clathrin transitions in vesicle formation. Overall, our findings indicate that clathrin dynamics and clathrin-coated vesicle formation can be modulated by the local physiological environment and represent an important regulatory mechanism.

流体剪切应力改变了内皮细胞中的凝集素动力学和囊泡形成。
内皮细胞(ECs)会经受各种高度动态的机械应力。其中,循环拉伸和质膜张力的增加会抑制非内皮细胞中凝集素介导的内吞(CME)。在这些生物物理上不利的条件下,ECs 如何维持 CME 仍然是个谜。此前,我们使用同步双波长轴向比率法(STAR)显微镜显示,静态培养的人脐静脉内皮细胞(HUVECs)和成纤维细胞样 Cos-7 细胞的内吞动力学相似。在此,我们询问血流产生的生物物理应力是否会影响 CME。我们使用我们的数据处理平台 DrSTAR 来研究 HUVECs 在经历流体剪切应力 (FSS) 之后是否会改变凝集素动态。我们发现,与静态对照组相比,在生理水平的 FSS 条件下培养的 HUVEC 具有更高的凝集素动态。FSS使凝集素包被囊泡的形成和非生产性扁平凝集素晶格分别增加了2.3倍和1.9倍。相对于流动刺激细胞中的凝集素招募,曲率阳性事件的曲率启动明显延迟,这突显了在囊泡形成过程中凝集素向扁平到弯曲的转变。总之,我们的研究结果表明,凝集素动力学和 CCV 的形成可受局部生理环境的调节,是一种重要的调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biophysical journal
Biophysical journal 生物-生物物理
CiteScore
6.10
自引率
5.90%
发文量
3090
审稿时长
2 months
期刊介绍: BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.
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