Characterization of ribostamycin and its impurities using a nano-quantity analyte detector: Systematic comparison of performance among three different aerosol detectors

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2024-09-01 Epub Date: 2024-06-05 DOI:10.1016/j.talanta.2024.126359

Fei Meng , Xiaohua Zhou , Yurong Hou , Haodong Zhao , Jinlin Zhang , Qing Huang , Mei Zhang , Erwin Adams , Yaozuo Yuan , Hai-Wei Shi

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Abstract

Characterization of aminoglycoside antibiotics like ribostamycin is important due to the complex composition and common toxic impurities. Aerosol detectors are often employed for determination of these non-absorbent analytes. In this work, a robust and cost-effective method was developed for simultaneous detection of ribostamycin and its related substances using high-performance liquid chromatography (HPLC) with a relative new aerosol detector named nano-quantity analyte detector (NQAD). With the introduction of less toxic but more compatible ion-pairs pentafluoropropionic acid (PFPA) and trifluoroacetic acid (TFA) in the eluent, an optimized separation effect was achieved. Compared with the other two aerosol detectors namely ELSD (evaporative light scattering detector) and CAD (charged aerosol detector), method verification and quantitative detection results revealed that NQAD had higher sensitivity than ELSD with a 0.8 μg/mL limit of detection, as well as wider linear range (from 2 μg/mL to 1000 μg/mL) than both CAD (from 2 μg/mL to 200 μg/mL) and ELSD (from 8 μg/mL to 200 μg/mL) detector. The performance of NQAD helped to realize detection of ribostamycin and its impurities with significant concentration differences in a single run. With a cation suppressor to eliminate the ion-suppression caused by the ion-pairs in the eluent, the structure of nine impurities in ribostamycin sample was characterized by liquid chromatography-mass spectrum (LC-MS). Both external standard and area normalization calculation were investigated, and NQAD obtained more accurate results due to its full-range linear response-to-concentration relationship, providing an alternative for routine quality control of multi analyte systems.

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使用纳米定量分析检测器表征核糖霉素及其杂质：三种不同气溶胶检测器性能的系统比较

由于核糖霉素等氨基糖苷类抗生素成分复杂，且常见有毒杂质，因此对其进行表征非常重要。通常采用气溶胶检测器来测定这些非吸收性分析物。本研究采用高效液相色谱法（HPLC）和一种名为 "纳米定量分析检测器（NQAD）"的新型气溶胶检测器，开发了一种稳健且经济有效的方法，用于同时检测核糖霉素及其相关物质。通过在洗脱液中引入毒性较低但相容性较好的离子对五氟丙酸（PFPA）和三氟乙酸（TFA），达到了优化的分离效果。方法验证和定量检测结果表明，与其他两种气溶胶检测器ELSD（蒸发光散射检测器）和CAD（带电气溶胶检测器）相比，NQAD的灵敏度比ELSD高，检出限为0.8 μg/mL，线性范围（从2 μg/mL到1000 μg/mL）比CAD（从2 μg/mL到200 μg/mL）和ELSD（从8 μg/mL到200 μg/mL）宽。NQAD 的性能有助于在一次运行中实现对浓度差异显著的核糖霉素及其杂质的检测。利用阳离子抑制器消除洗脱液中离子对造成的离子抑制，通过液相色谱-质谱（LC-MS）对核糖霉素样品中的九种杂质进行了结构表征。研究了外标法和面积归一化计算方法，结果表明 NQAD 具有全范围的线性响应与浓度关系，可获得更准确的结果，为多分析物系统的常规质量控制提供了一种替代方法。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.