Optimization of Extraction of Four Components from Radix Scrophulariae with Natural Deep Eutectic Solvents and Evaluation of Extract's Antioxidant Activity.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2025-01-14 DOI:10.1093/chromsci/bmae037

Ping Huang, Yanxia Zhou, Fei Ruan, Jianyu Sun, Jinglin Shen, Hongmei Chen

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Abstract

In this research, eight natural deep eutectic solvents (NaDESs) consisting of food-grade ingredients were screened for the extraction of four bioactive compounds (acteoside, cinnamic acid, angoroside C and harpagoside) from radix scrophulariae (RS). Among these NaDESs, Proline-Glycerol NaDES with higher comprehensive score was selected. The Criteria Importance Through Intercriteria Correlation (CRITIC) was applied to calculate the information entropy and the weight of indexes, and figured out a comprehensive score. The weights of acteoside, cinnamic acid, angoroside C and harpagoside were 0.369, 0.172, 0.241 and 0.218, respectively. Response surface methodology (RSM) mathematical model was used to optimize the extraction parameters. The optimal extraction parameters were as follows: extraction time with 42.21 min, NaDES concentration with 52.89%, solid-to-liquid ratio with 1 : 37.05 g/mL and the predicted value of comprehensive score was 0.885. Under the optimal condition, the comprehensive score was 0.903 ± 0.005. Finally, the antioxidant activity experiment revealed that the 1,1-Diphenyl-2-picrylhydrazyl · radical scavenging activity and hydroxyl radical scavenging activity of the extract at 2.0 mg/mL and 1.5 mg/mL were approximately equal to those of ascorbic acid, respectively. The results showed that the extraction condition optimized by RSM combined with CRITIC was reasonable and dependable, and the extract of radix scrophulariae exhibited good antioxidant activity.

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用天然深共晶溶剂优化萃取樟芝中的四种成分并评估萃取物的抗氧化活性

本研究筛选了八种由食品级成分组成的天然深共晶溶剂（NaDESs），用于从樟芝（RS）中提取四种生物活性化合物（放线菌苷、肉桂酸、血管苷 C 和哈巴苷）。在这些 NaDES 中，脯氨酸-甘油 NaDES 的综合得分较高。应用 "标准重要性与标准间相关性"（CRITIC）计算信息熵和指标权重，得出综合得分。结果表明，猕猴桃苷、肉桂酸、血管苷 C 和哈巴苷的权重分别为 0.369、0.172、0.241 和 0.218。采用响应面法（RSM）数学模型对提取参数进行了优化。最佳萃取参数为：萃取时间为 42.21 分钟，NaDES 浓度为 52.89%，固液比为 1 : 37.05 g/mL，综合得分预测值为 0.885。在最佳条件下，综合得分为 0.903 ± 0.005。最后，抗氧化活性实验表明，在 2.0 毫克/毫升和 1.5 毫克/毫升条件下，提取物的 1,1-二苯基-2-苦基肼自由基清除活性和羟自由基清除活性分别与抗坏血酸大致相当。结果表明，RSM结合CRITIC优化的提取条件合理可靠，蟾酥提取物具有良好的抗氧化活性。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.