Transcriptional regulators with broad expression in the zebrafish spinal cord

IF 2 3区生物学 Q2 ANATOMY & MORPHOLOGY

Developmental Dynamics Pub Date : 2024-06-08 DOI:10.1002/dvdy.717

Samantha J. England, Paul C. Campbell, Santanu Banerjee, Richard L. Bates, Ginny Grieb, William F. Fancher, Katharine E. Lewis

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Abstract

Background

The spinal cord is a crucial part of the vertebrate CNS, controlling movements and receiving and processing sensory information from the trunk and limbs. However, there is much we do not know about how this essential organ develops. Here, we describe expression of 21 transcription factors and one transcriptional regulator in zebrafish spinal cord.

Results

We analyzed the expression of aurkb, foxb1a, foxb1b, her8a, homeza, ivns1abpb, mybl2b, myt1a, nr2f1b, onecut1, sall1a, sall3a, sall3b, sall4, sox2, sox19b, sp8b, tsc22d1, wdhd1, zfhx3b, znf804a, and znf1032 in wild-type and MIB E3 ubiquitin protein ligase 1 zebrafish embryos. While all of these genes are broadly expressed in spinal cord, they have distinct expression patterns from one another. Some are predominantly expressed in progenitor domains, and others in subsets of post-mitotic cells. Given the conservation of spinal cord development, and the transcription factors and transcriptional regulators that orchestrate it, we expect that these genes will have similar spinal cord expression patterns in other vertebrates, including mammals and humans.

Conclusions

Our data identify 22 different transcriptional regulators that are strong candidates for playing different roles in spinal cord development. For several of these genes, this is the first published description of their spinal cord expression.

Abstract Image

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斑马鱼脊髓中广泛表达的转录调节因子

背景：脊髓是脊椎动物中枢神经系统的重要组成部分，它控制运动并接收和处理来自躯干和四肢的感觉信息。然而，我们对这一重要器官的发育过程还有很多不了解的地方。在这里，我们描述了 21 个转录因子和一个转录调节因子在斑马鱼脊髓中的表达：野生型和 MIB E3 泛素蛋白连接酶 1 斑马鱼胚胎中的 sox2、sox19b、sp8b、tsc22d1、wwdhd1、zfhx3b、znf804a 和 znf1032。虽然所有这些基因都在脊髓中广泛表达，但它们的表达模式各不相同。一些基因主要在祖细胞领域表达，另一些则在有丝分裂后细胞亚群中表达。鉴于脊髓发育以及协调脊髓发育的转录因子和转录调节因子的保守性，我们预计这些基因在其他脊椎动物（包括哺乳动物和人类）中也会有类似的脊髓表达模式：我们的数据确定了 22 种不同的转录调节因子，它们是在脊髓发育过程中发挥不同作用的有力候选者。结论：我们的数据发现了 22 种不同的转录调节因子，它们是在脊髓发育过程中扮演不同角色的有力候选者。对于其中的几个基因，这是首次发表关于它们在脊髓表达的描述。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental Dynamics 生物-发育生物学

CiteScore

5.10

自引率

8.00%

发文量

116

审稿时长

3-8 weeks

期刊介绍： Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.