Elevated proinflammatory cytokines in response to mechanical stimulus are associated with reduced knee loading 2 years after anterior cruciate ligament reconstruction

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Arielle G. Fischer , Matthew R. Titchenal , Eleonora Migliore , Jessica L. Asay , Jennifer C. Erhart-Hledik , Thomas P. Andriacchi
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Abstract

Background

The aim of this study was to test the hypothesis that proinflammatory cytokines correlate with knee loading mechanics during gait following a mechanical walking stimulus in subjects 2 years after anterior cruciate ligament reconstruction. Elevated systemic levels of proinflammatory cytokines can be sustained for years after injury. Considering roughly 50% of these patients progress to Osteoarthritis 10–15 years after injury, a better understanding of the role of proinflammatory cytokines such as tumor necrosis factor-α and Interleukin-1β on Osteoarthritis risk is needed.

Methods

Serum proinflammatory cytokines concentrations were measured in 21 subjects 2 years after unilateral ACLR from blood drawn at rest and 3.5 h after 30 min of walking. An optoelectronic system and a force plate measured subjects' knee kinetics. Correlations were tested between inflammatory marker response and knee extension and knee adduction moments.

Findings

Changes in proinflammatory cytokines due to mechanical stimulus were correlated (R = 0.86) and showed substantial variation between subjects in both cytokines at 3.5 h post-walk. Knee loading correlated with 3.5-h changes in tumor necrosis factor-α concentration (Knee extension moment: R = -0.5, Knee adduction moment: R = -0.5) and Interleukin-1β concentration (Knee extension moment: R = -0.44). However, no significant changes in concentrations were observed in tumor necrosis factor-α and Interleukin-1β when comparing baseline and post walking stimulus conditions.

Interpretation

The significant associations between changes in serum proinflammatory markers following a mechanical stimulus and gait metrics in subjects at risk for developing Osteoarthritis underscore the importance of investigating the interaction between biomarkers and biomechanical factors in Osteoarthritis development.

前交叉韧带重建 2 年后,对机械刺激做出反应的促炎细胞因子升高与膝关节负荷减轻有关
背景本研究的目的是检验前交叉韧带重建 2 年后的受试者在机械行走刺激下步态期间促炎细胞因子与膝关节负荷力学相关性的假设。促炎细胞因子的全身水平升高可在受伤后持续数年。考虑到这些患者中约有 50% 在受伤 10-15 年后发展为骨关节炎,因此需要更好地了解肿瘤坏死因子-α 和白细胞介素-1β 等促炎细胞因子对骨关节炎风险的作用。方法:对 21 名受试者进行血清促炎细胞因子浓度测量,这些受试者在单侧前交叉韧带重建 2 年后,分别在休息时和行走 30 分钟后 3.5 小时抽血。光电系统和测力板测量了受试者的膝关节运动能力。研究结果机械刺激引起的促炎细胞因子的变化具有相关性(R = 0.86),并且在步行后 3.5 小时两种细胞因子的变化在受试者之间存在很大差异。膝关节负荷与 3.5 小时后肿瘤坏死因子-α 浓度(膝关节伸展力矩:R = -0.5,膝关节内收力矩:R = -0.5)和白细胞介素-1β 浓度(膝关节伸展力矩:R = -0.44)的变化相关。在机械刺激后血清促炎标志物的变化与骨关节炎高危人群的步态指标之间的显著关联强调了研究骨关节炎发展过程中生物标志物与生物力学因素之间相互作用的重要性。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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