Anne Calvel;Olivia Peytral-Rieu;Marie-Pierre Rols;David Dubuc;Katia Grenier
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引用次数: 0
Abstract
This letter presents a biosensor first developed to perform microwave dielectric spectroscopy (MDS) analysis, which is evaluated for in situ electroporation (EP) at the single-cell level. After demonstrating the possibility to characterize living cancer cells in the radio-frequency (RF) sensor by acquiring their dielectric properties, we then evaluate the use of this RF sensor to apply high electric field stimulus to trigger cell permeabilization. The impact of applied voltage and therefore electric field intensity on the number of permeabilized cells is defined, leading to the establishment of an EP threshold, which is EP conditions and cell dependent. This paves the way for further studies assessing the impact of in situ EP using the MDS method performed with a unique microdevice and consequent observation and understanding of the EP phenomenon.
本文首先介绍了一种用于进行微波介电光谱(MDS)分析的生物传感器,并对其进行了单细胞水平的原位电穿孔(EP)评估。通过获取活癌细胞的介电特性,我们展示了在射频(RF)传感器中描述其特征的可能性,然后我们评估了使用这种射频传感器施加高电场刺激以触发细胞渗透的情况。我们确定了外加电压和电场强度对通透细胞数量的影响,从而确定了 EP 阈值,该阈值取决于 EP 条件和细胞。这为进一步研究使用独特的微型设备执行 MDS 方法评估原位 EP 的影响以及随后观察和了解 EP 现象铺平了道路。