Effects of batch and continuous-flow operation on biotreatment of Mn(II)-containing mine drainage

IF 5.9 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Obey Gotore , Miho Watanabe , Kunihiro Okano , Naoyuki Miyata , Taiki Katayama , Tetsuo Yasutaka , Yuki Semoto , Takaya Hamai
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引用次数: 0

Abstract

The biotreatment of mine drainage containing dissolved manganese (Mn) using Mn(II)-oxidizing bacteria is challenging. Sequencing-batch (SBRs) and continuous-flow reactors (CFRs) packed with limestones and inoculated with the mine-drainage microbial community were compared to determine the removal efficiency of Mn(II) from mine drainage. Mn(II) removal in CFRs was 11.4%±0.0% (mean ± standard deviation) in the first two weeks and; it slightly increased to 13.6%±0.0% after four weeks, and more than 94% of Mn(II) was removed under the steady-state treatment phase. The performance of SBRs was more effective, wherein 24.4%±0.1% of Mn was removed in the first two weeks, and in four weeks, surpassed 66.6%±0.2%. Rapid Mn(II) removal observed in the start-up of SBR resulted from higher microbial metabolic activities. The adenosine triphosphate (ATP) content of the microbial community was four-fold more than in CFR, but comparable during the steady-state phase. The Mn-oxide deposits occurring in the SBR and CFR at steady-state were mixed phases of birnessite and woodruffite, and the average Mn oxidation valence in the SBR (+3.73) was slightly higher than that in the CFR (+3.54). During the start-up treatment, the closest relatives of Methyloversatilis, Methylibium, and Curvibacter dominated the SBR, whereas putative Mn oxidizers were associated with Hyphomicrobium, Pedobacter, Pedomicrobium, Terricaulis sp., Sulfuritalea, and Terrimonas organisms. The growth of potential Mn-oxidizing genera, including Mesorhizobium, Rhodococcus, Hydrogenophaga, Terricaulis sp., and ‘Candidatus Manganitrophus-noduliformans’ was observed under the steady state. The SBR operation was effective as a prior start-up treatment for mine drainage-containing Mn(II), through which the CFR performed well as posterior bio-treatment.

Abstract Image

分批和连续流操作对含锰(II)矿井排水生物处理的影响
利用锰(II)氧化细菌对含有溶解锰(Mn)的矿井排水进行生物处理具有挑战性。为了确定矿井排水中锰(II)的去除效率,我们对装有石灰石并接种了矿井排水微生物群落的序批式反应器(SBR)和连续流反应器(CFR)进行了比较。前两周,CFR 对锰(II)的去除率为 11.4%±0.0%(平均值±标准偏差),四周后略有上升,达到 13.6%±0.0%,在稳态处理阶段,锰(II)的去除率超过 94%。SBR 的性能更为有效,前两周的锰去除率为 24.4%±0.1%,四周后超过 66.6%±0.2%。在 SBR 启动过程中观察到的 Mn(II) 快速去除是由于微生物代谢活动较高所致。微生物群落的三磷酸腺苷 (ATP) 含量是 CFR 的四倍,但在稳态阶段与 CFR 相当。在稳态阶段,SBR 和 CFR 中出现的氧化锰沉积物为桦木石和木屑石的混合相,SBR 中的平均氧化锰价(+3.73)略高于 CFR 中的氧化锰价(+3.54)。在启动处理过程中,Methyloversatilis、Methylibium 和 Curvibacter 的近亲在 SBR 中占主导地位,而推定的锰氧化剂则与 Hyphomicrobium、Pedobacter、Pedomicrobium、Terricaulis sp.、Sulfuritalea 和 Terrimonas 生物有关。在稳定状态下,观察到了潜在锰氧化菌属的生长,包括 Mesorhizobium、Rhodococcus、Hydrogenophaga、Terricaulis sp.和 "Candidatus Manganitrophus-noduliformans"。SBR 操作作为含锰(II)矿井排水的先期启动处理是有效的,通过该操作,CFR 作为后置生物处理效果良好。
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来源期刊
Journal of Environmental Sciences-china
Journal of Environmental Sciences-china 环境科学-环境科学
CiteScore
13.70
自引率
0.00%
发文量
6354
审稿时长
2.6 months
期刊介绍: The Journal of Environmental Sciences is an international journal started in 1989. The journal is devoted to publish original, peer-reviewed research papers on main aspects of environmental sciences, such as environmental chemistry, environmental biology, ecology, geosciences and environmental physics. Appropriate subjects include basic and applied research on atmospheric, terrestrial and aquatic environments, pollution control and abatement technology, conservation of natural resources, environmental health and toxicology. Announcements of international environmental science meetings and other recent information are also included.
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